摘要
目的:基于一贯性高血糖症能导致胰岛素抵抗的假说,我们研究了高糖低脂饮食导致的非胰岛素依赖糖尿病大鼠的生理功能和组织学改变。方法:取正常新西兰兔24只,随机分为正常组、糖尿病(DM)组、糖尿病骨钙素干预(DM+OCGY)组。采用高唐低脂饮食喂养新西兰兔,建立非胰岛素依赖糖尿病模型。建立模型后,DM+OCGY组腹腔注射骨钙素(2.5 mg/kg·d),DM组腹腔注射相等量生理盐水。一个月后处死新西兰兔。用HE染色方法检测新西兰兔胰腺形态改变。结果:高糖低脂饮食能有效引起新西兰兔血糖的升高,尿糖阳性,HE染色表明胰腺细胞发生相应改变,DM+OCGY组空腹血糖的浓度明显降低,能逆转胰腺的相关改变。结论:高糖低脂饮食能诱导非胰岛素依赖糖尿病的发生,骨钙素对非胰岛素依赖型糖尿病血糖浓度有一定的调节作用。
Objective： Based on the hypothesis that consistent hyperglycemia can result in insulin resistance, we explored the induction of non-insulin dependent diabetes mellitus （NIDDM） using diet of high glycemic/low fat index and compared the effects on the physiology and histology of the rats. Methods： The 24 normal New Zealand rats were randomly divided into three groups, the normal group, the diabetes mellitus group（DM） and the osteocalcin treated diabetes mellitus group（DM＋OCGY）. After the non-insulin dependent diabetes mellitus model were established by using diet of high glycemic/low fat, DM＋OCGY group were induced by single intraperitoneal injection of osteocalcin 2.5mg/kg. d, and the DM group were intraperitoneally injected with equal normal saline. The rats were sacrificed one month later. HE staining method was used to detect the pancreas changes of the rats. Results： The high glycemic/low fat diet could cause the increase of blood sugar level of New Zealand rat. The glycosuria presented to be positive, and the HE staining results showed there were corresponding changes in pancreatic cells. The fasting blood-glucose level was decreased in DM＋OCGY group, could reverse some related changes of pancreatic cells. Conclusion： The high glycemic/low fat diet could induce the development of non-insulin dependent diabetes mellitus. Osteocalcin had some regulating effects on the blood-glucose level of non-insulin dependent diabetes mellitus rats.
出处
《现代生物医学进展》
CAS
2012年第33期6443-6446,共4页
Progress in Modern Biomedicine
关键词
糖尿病
高脂饮食
骨钙素
HE染色
Diabetes mellitus
High fat diet
Osteocalcin
HE staining
