摘要
目的 为寻找有效的抗日本血吸虫感染疫苗候选抗原分子。方法 用紫外线照射致弱尾蚴免疫兔血清筛选日本血吸虫成虫cDNA文库 ,再用同法免疫鼠脾细胞制备的单克隆抗体对所获阳性克隆进行再筛选 ,并对免疫筛选的阳性克隆的cDNA插入片段进行PCR扩增。结果 兔免疫血清确定 12个阳性克隆 ,其中 6个克隆与 2株以上的单抗呈阳性反应 :PCR扩增cDNA插入片段大小约 40 0bp～ 1 4kb左右。结论 已筛选到与致弱尾蚴免疫兔血清及同法免疫制备单抗产生特异反应的阳性克隆 ,获得一批编码可能具有保护性作用的日本血吸虫抗原的基因片段。
Aim To develop recombinant vaccine candidate antigens Methods S japonicum adult cDNA library was screened using sera from immune rabbits vaccinated with irradiated cercariae and also using monoclone antibodies(McAbs)Against membrane antigen of S japonicum schistosomula The inserts of six positive clones both to the sera and McAbs were specifically amplified by PCR Results Twelve positive clones were obtained with immune rabbits sera and among those clones,six clones were reacted with at least two strains of four McAbs The size of these inserts ranged from 400bp to 1 4kp Conclusions The positive clones were found to be related to protective immunity,and they may be genes encoding protective S japonicum antigens
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第3期31-33,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金
安徽省自然科学基金!资助项目 (No39570 646
96 -医 - 2 8)
