摘要
目的探讨姜黄素及其联合顺铂对体外培养人卵巢癌细胞株SKOV3增殖的抑制作用。方法将培养的人卵巢癌SKOV3细胞分别单独(姜黄素组或顺铂组)及联合(姜黄素与顺铂联用组)加入不同浓度姜黄素(5、10、20、40μmol/L)、顺铂(2.5 mg/L)作用后,采用四甲基偶氮唑盐比色法(MTT法)检测SKOV3细胞增殖情况。结果不同浓度姜黄素组随着药物浓度增加、作用时间延长,细胞的增殖抑制率上升,差异有统计学意义(P<0.05);联合用药组明显高于单一用药组(P<0.05),两药联合应用具有协同作用。结论姜黄素对卵巢癌SKOV3细胞增殖具有抑制作用,在一定范围内,这种抑制作用呈剂量-时间依赖性;姜黄素与顺铂联合应用具有协同作用,可提高SKOV3细胞对顺铂的敏感性,是一种理想的化疗增敏剂。
Objective To investigate the proliferation-inhibiting effect of curcumin and curcumin combined with cisplatin on human ovarian cancer cell line SKOV3 in vitro. Methods Human ovarian cancer SKOV3 cells were cultured and treated with different concentrations of eurcumin （5,10,20,40 μmol/L）, cisplatin （2.5 mg/L）, or curcumin combined with eisplatin in different concentrations. The proliferation of SKOV3 ceils was detected by MTT assay. Results MTT assay showed that curcumin with different concentrations could significantly inhibit the proliferation of SKOV3 cells in time-dependent and dose-dependent manners （P〈0.05）. Compared with that in single drug groups, the inhibition effect in the combination treatment group was significantly higher（P〈0.05） ,showing the synergic effect. Conclusion Curcumin can inhibit the proliferation of SKOV3 ceils. In a certain range, the effect is in dose- and time-dependent manners. Curcumin combined with cisplatin has the synergistic effect can inrease the SKOV3 cell＇s sensitivity to cisptin, and is an ideal sensitivity-increasing agent for chemotherapy.
出处
《现代医药卫生》
2012年第23期3523-3524,共2页
Journal of Modern Medicine & Health
基金
川北医学院附属医院科研基金资助项目(2010-院内-11)
