摘要
目的探讨103钯(103pd)放射性支架对p53抑癌基因在辐射诱导胆管癌细胞凋亡中的影响和意义。方法 12只裸鼠随机分2组,每组6只,在裸鼠胆管癌内分别植入103pd放射性支架和普通胆管支架,术后30 d取出胆管癌标本进行HE染色和p53基因原位杂交,DAB染色p53基因表达阳性者细胞质呈棕黄色,胆管癌细胞凋亡染色细胞核中有棕黄色浓染的为阳性细胞。结果103pd放射性支架组胆管癌细胞p53基因阳性表达率为89.63%,而普通支架组为21.76%,2组比较差异有统计学意义(P<0.01);103pd放射性支架组胆管癌凋亡细胞数明显大于普通支架组,2组比较差异有统计学意义(P<0.05)。结论103pd放射性支架可以激活p53基因,使其功能提高,诱导胆管癌细胞凋亡。
Objective To investigate the role of tumor suppressor genes （p53 gene） in radioactive induced cholangioarcinoma cells apoptosis. Methods A total of 12 nude mice were randomly divided into 103pd radioactive stent group （n =6） and control group （n =6）. Cross-sections were stained by HE for cholangiocarcinoroa. The expression of p53 in cholangiocarcinoma tissue was detected using in situ hy- bridization,cells with the brow-yellow nucleus ＇after DAB coloration indicates positive staining. Positive rate was analyzed under micrescope and apoptosis cell in paraffln-bedding cholangiocarcinoma tissue section was determined using immunohistological staining method. Results Expression of p53 was higher in the 103pdradioactive stem group than that in the control group （P 〈 0.01 ）. The cholangiocarcinoma cell apoptosis rate was significantly increased in the 103pd radioactive stent group than the control group （P 〈 0.01 ）. Conclusion These results suggest that p53 gene was increased by 103pd and induced cholangicareinoma cell apoptosis,the results ~so show that 103pd radioactive stent can inhibit cholangiocarcinoma cell proliferation.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2012年第12期1099-1101,共3页
Journal of China Medical University
基金
辽宁省自然科学基金资助项目(20042063)
辽宁省教育厅高校科研计划(05L445)
辽宁省科技计划项目(2006225001-6)
沈阳市科技计划项目(F12-193-9-01)
