摘要
为解决杭菊的品种退化问题,以大白菊、小白菊、黄菊三个品种类型的茎尖为外植体,接种在不同pH的MS培养基及不同激素配比的培养基上。结果表明,pH=5.8～6.4时外植体诱愈率在80%以上;外植体接种在不同激素配比的MS培养基上,NAA为0.2 mg/L时KT的诱愈率明显高于6-BA。诱导生根的适宜培养基是1/2 MS+NAA 0.2 mg/L。
To solve the breed degeneration of Chrysanthemum the stem apex of Chrysanthemum morifolium used as explants were inoculated on MS medium with different pH and supplemented with different ratio of plant hormone.The results showed that when pH was between 5.8 and 6.4,the rate of callus were all over 80%.While explants inoculated on MS medium supplemented with NAA 0.2 mg/L and KT 0.1～0.2 mg/L,the rate of callus was noticeably higher than that of NAA 0.2 ng/L and 6-BA 1 3 mg/L.The preferable medium which roots could be induced was 1/2 MS + NAA 0.2 mg,/L.
出处
《中药材》
CAS
CSCD
2000年第3期125-127,共3页
Journal of Chinese Medicinal Materials
关键词
杭菊花
茎尖
组织培养
Chrysanthemum morifolium
Stem apex
Tissue culture
