摘要
为获得与建鲤增重相关的分子标记进行建鲤分子育种,实验在建鲤心肌型脂肪酸结合蛋白(FABP)基因上筛选了与增重相关的SNP位点。首先使用PCR扩增到2个建鲤FABP3基因,jlFABP3a和3b基因,两个基因均有4个外显子和3个内含子组成,3a和3b阅读框相似性为93%,编码133个氨基酸,相似性为94%,内含子长度和序列存在着明显差异。jlFABP3的系统进化和传统的分类地位一致。通过序列比对,在3a和3b上分别找到26和25个SNP位点。构建PCR-RFLP法检测了其中3个SNPs在建鲤群体中的分布,并与增重进行相关分析,结果显示,C30G与检测群体雌、雄鱼鱼种阶段和雌鱼成鱼阶段增重显著相关(P<0.05),CC型个体增重显著快于CG型个体。G267T在5个家系中与雌鱼成鱼增重相关,在7个家系中不同基因型个体增重虽呈相同趋势但差异不显著(P>0.05)。同时考虑G267T和C30G位点,则发现GGCC个体在雌、雄鱼增重均最大,在雌、雄鱼中分别比GGCG个体快17%和12%。GGCC个体在实验样本中占约9%,存在着较大的选育空间。
Fatty Acid Binding Proteins are mainly involved in fatty acid transfer in the extracellular and intracellular membrane,and also take part in the regulation of cell growth and proliferation.In this experiment,two heart and muscle fatty acid binding protein genes,jlFABP3a and 3b,were isolated from C.carpio var.jian.Consistent with other FABP3s,jlFABP3s contain four exons and three introns.The open reading frames of 3a and 3b encode 133 amino acids and share 93% and 94% similarity with each other in DNA and protein level,respectively.And there was clear difference in sequences and lengths between two genes introns.Twenty-six and twenty-five SNPs were found on jlFABP3a and 3b respectively in C.carpio var.jian population using alignment sequences from different individuals.And genotypes in 3 SNP loci were detected using PCR-RFLP.The SNP-weight gain correlation analysis indicated C30G was significantly associated with male juvenile,female juvenile and adult fish weight gain（P0.05） in total experimental samples.Individuals with CC genotype grew faster than CG individuals.G267T was significantly correlated with female adult fish weight gain（P0.05） in 5 families,and three genotype individuals had same growth trends in the 7 populations,though not significant（P0.05）.Taking two SNPs（G267T and C30G） into account,correlation analysis revealed individuals with GGCC grew the fastest in female and male fish,and grew 17% and 12% faster than GGCG ones.In the experimental samples,the GGCC individuals account for only about 9%,indicating the presence of large selection space,and can be used as reference in molecular assistant breeding of C.carpio var.jian.
出处
《水产学报》
CAS
CSCD
北大核心
2012年第12期1809-1818,共10页
Journal of Fisheries of China
基金
水产分子育种共性技术的建立与应用(200903045)
国家“八六三”高技术研究发展计划(2011AA100401)
现代农业产业技术体系建设专项(CARS-46)
关键词
建鲤
心肌型胞内脂肪酸结合蛋白
单核苷酸多态性
SNP-增重相关分析
Cyprinus carpio var.jian
heart and muscle fatty acid binding protein
single nucleotide polymorphism
SNP-weight gain correlated analysis
