摘要
采用毛细管区带电泳方法对抗心律失常新药氯苄律定及其合成前体盐酸黄连素和四氢黄连素进行了系统分离，以８０ ｍｍｏｌ／Ｌ磷酸盐缓冲液（ｐＨ ２．５）－乙醇（５：１，Ｖ／ Ｖ）为背景电介质，操作电压 １０ ｋＶ，毛细管柱温 ２５℃，电迁移进样 ４ ｋＶ ｘ ６ ｓ，柱上 ２３０ ｎｍ检测，盐酸黄连素、四氢黄连素、氯苄律定及内标物盐酸奎宁可在 ２０ ｃｍ ｘ ２５ μｍ涂层毛细管上 ６．０ ｍｉｎ之内实现基线分离。对优选的分离条件进行方法耐用性评价，其中乙醇含量和毛细管柱温显著影响电泳分离结果。
The optimal separation of antiarrhythmic new drug chlorobenzyltetrahydroberberine (CBTHB ) and its related substances berberine hydrochloride (B) and tetrahydroberberine (THB ) by capillary electrophoresis was reported. The effect of main operating parameters on the migration behaviour of analytes was discussed systematically. In a coated silica capillary (20 cm x 25 μm), the berberine hydrochloride, tetrahydroberberine, chlorobenzyltetrahydroberberine and internal standard quinine dihydrochloride could be separated within 6. 0 ruin in the following optimum conditions: 80 mmol/L phosphate buffer at pH 2. 5 containing 20% ethanol, 10 kV applied 'voltage, electromigration injection 4 kV x 6 s, 230 nm on-line detection and 25℃ capillary temperature. The ruggedness test was evaluated based on variations of buffer pH value, ethanol content and capillary temperature, and the factors of ethanol content and capillary temperature would effect significantly electrophoretic separation.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第7期868-871,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金!(No.39700181)
关键词
毛细管电泳
分离
氯苄律定
方法耐用性评价
Capillary zone electrophoresis
berberine hydrochloride
tetrahydroberberine
chlorobenzyltetrahydroberberine
method ruggedness
