摘要
建立了高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)测定大豆粕、大豆低聚糖浆样品中葡萄糖、果糖、蔗糖、蜜二糖、半乳糖、棉籽糖和水苏糖7种糖的方法。选用Dionex CarboPac PA20阴离子交换柱,以0.25mol/L NaOH和水为淋洗液进行梯度洗脱,考察了色谱柱温度和NaOH浓度对各种糖分离的影响,确定最佳柱温为20℃,7种糖可以在30min内实现基线分离。7种糖的检出限(S/N=3)为2～9μg/L,具有较宽的线性范围(0.1～80.0mg/L)。大豆粕样品测定的相对标准偏差在2.67%～4.25%之间,7种糖的加标回收率在91%～108%之间。该方法简便易行,灵敏度高,重现性良好,适用于大豆粕和大豆低聚糖浆样品中的糖组分的分析。
A chromatographic method was developed for the determination of fructose, galactose, glucose, sucrose,melibiose,raffinose and stachyose in soybean meal and soy oligosaccharide samples using high performance anion-exchange chromatography coupled with pulsed amperometric detection （HPAEC- PAD）. The effect of columns, column temperature and concentrations of NaOH solution on separation efficiency was investigated. The optimal conditions were carried out on Dionex CarboPac PA 20 anionexchange chromatography with 0.25 mol/L NaOH and water as gradient eluents at 20 ℃. The detection limits（20 uL injection,S/N=3） ranged from 2 to 9 ug/L,with a wide linear range of 0.1-80.0 mg/L. The relative standard deviation of the soybean meal samples ranged between 2.67% and 4. 25% ,and the recoveries were 92.1%-108.0%. The method is suitable for determination of common saccharides in soybean meal and soy oligosaccharides samples for its simpleness and high sensitivity as well as good reproducibility.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第6期843-846,共4页
Journal of Analytical Science
关键词
离子色谱法
脉冲安培检测
大豆粕
大豆低聚糖
糖
Ion chromatography
Pulsed amperometric detection
Soybean meal
Soy oligosaccharides
Saecharides
