摘要
目的探讨偏侧咀嚼对大鼠咬肌线粒体的影响,进一步研究偏侧咀嚼致咬肌功能紊乱的机制。方法用电镜观察线粒体形态和数目的变化并计算线粒体损伤指数;用原子分光光度法检测Ca2+含量;用分光光度法检测线粒体渗透性转换孔道(MPTP)的开放程度。结果实验组拔牙侧线粒体损伤指数评分均低于实验组非拔牙侧,并且明显低于对照组(P<0.01),其中,4周组评分最低。除8周组以外实验组拔牙侧Ca2+含量均升高,且明显高于对照组(P<0.05)。其中,4周组Ca2+含量升高最为明显。实验组拔牙侧线粒体渗透性转换孔道的开放程度均大于实验组非拔牙侧和对照组(P<0.05),6周组达到最高。线粒体渗透性转换孔的开放程度与线粒体损伤程度呈负线性相关(r=-0.74,P<0.05)。结论高含量Ca2+激活了线粒体通透性转换孔开放的相关信号通路,引起线粒体损伤和细胞凋亡,这可能是偏侧咀嚼致咬肌功能紊乱的发生机制之一。
Objective To explore the effect of hemimastication on the mitochondria of rat masseter muscle and to study the mechanism of masseter muscle dysfunction induced by hemimastication. Methods Mitochondrial morphology and number changes were observed by electron microscope, and the mitochondrial damage index was measured; Ca2＋ con- tent was measured with atomic absorption spectrometry; The mitochondrial permeability transition pore（MPTP） opening was detected by UV spectrophotometry. Results The mitochondrial damage index score of the extraction side was low- er than that of the non-extraction side, and significantly lower than that of the control group（P 〈0.01 ）. The 4-week group had the lowest score. Compared with those of the control groups, Ca2＋ contents in the experimental groups had the higher level except the g-week group （ P 〈 0.05 ）. The opening of mitochondrial permeability transition pore had a higher level on the extraction side than that on the non-extraction side and the control group （ P 〈 0.05 ）. The 6-week group had the highest level. The opening of mitochondrial permeability transition pore was negatively correlated to mito- chondrial damage index （ r = - 0. 74, P 〈 0.05 ）. Conclusion The signal way of the mitochondrial permeability transi- tion pore opening can be activated by high concentration of calcium, which may cause the damage of mitochondria and apoptosis. This pathway may play an important role in the mechanism that hemimastication results in masticatory mus- cles dysfunction.
出处
《山东大学学报（医学版）》
CAS
北大核心
2012年第12期52-55,60,共5页
Journal of Shandong University:Health Sciences
