摘要
目的研究难治性癫痫患者及皮罗卡品致痫大鼠脑组织中PTPRT的表达，以探讨难治性癫痫可能的发病机制。方法用免疫组织化学、免疫荧光、Western blot法检测胛PRT在难治性癫痫患者及癫痫发作后6h及1、7、14、30、60d的匹罗卡品致痫大鼠脑组织中的表达，并分别与无癫痫发作的人以及大鼠的脑组织比较。结果在人颞叶皮质中，PTPRT主要在对照组和病例组的神经元有表达，PTPRT在难治性癫痫患者（0．277±0．048）脑组织中比对照组（0．171±0．025）显著升高（t=9．586，P〈0．05）。在大鼠颞叶脑组织中，对照组和实验组的PTPRT主要在神经元中表达。mRT在实验组颞叶中的表达与对照组相比，在癫痫发作后24h内不断升高，7d和14d后表达水平则下降，30d和60d后表达又升高（4比值：对照组0．443±0．039，6h0．840±0．032，24h1．113±0．064，7d0．564±0．039，14d0．570±0．029，30d0．899±0．034，60d1．011±0．074，F=256．427，P〈0．05）。结论通过对PTPRT在癫痫动物模型和癫痫患者颞叶脑组织表达规律的研究，结合PTPRT的功能我们推测PTPRT可能通过突触重构以及苔藓纤维出芽参与了神经网络的重构，从而导致了难治性癫痫的发生。
Objective To investigate the expression changes of the protein tyrosine phosphatase receptor T （PTPRT） in temporal lobe epileptic foei in the experimental animals and epileptic patients and the relationship between PTPRT and epileptogenesis. Methods After getting the epilepsy lobe tissue from the experimental and control groups, immunohistochemistry, immunofluorescence and Western blot analysis were used to assess the expression of PTPRT and its changes. Results In the temporal lobe tissue of intractable patients and control group, PTPRT was mainly expressed in the neurons. PTPRT was significantly increased in patients with intractable epilepsy （0. 277±0. 048 ） than that in the control group （0. 171±0. 025 ; t = 9. 586, P 〈 0. 05 ）. PTPRT was mainly expressed in the neurons in the temporal lobe brain tissue of the rats in the control group and experimental group. Compared with control group, the expression of PTPRT in the temporal lobe tissue was increased within 24 h post-seizure, and decreased 1 and 2 weeks post-seizure, then it was increased 1 and 2 months post-seizure （A ratio： control 0. 443 ± 0. 039,6 h 0.840±0.032,24 h 1. 113 ±0.064,7 d 0.564 ±0.039,14 d 0.570 ±0.029,30 d 0.899 ±0.034,60 d 1.011 ±0.074, F = 256.427,P 〈 0.05）. Conclusions Through researches into the expression and function of PTPRT in the temporal lobe brain tissue of patients with intractable epilepsy and animal models, we presume that the PTPRT plays a role in the synapses reorganization and mossy fiber sprouting, and participates in the reconstruction of the neural network which leads to the intractable epilepsy.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2012年第12期883-887,共5页
Chinese Journal of Neurology
关键词
癫痫
颞叶
受体样蛋白质酪氨酸磷酸酶类
2类
苔藓纤维
海马
疾病模型
动物
Epilepsy, temporal lobe
Receptor-like protein tyrosine phosphatases, class 2
Mossy fibers, hippoeampal
Temporal lobe
Disease models, animal
