摘要
通过结合TLC、UV及三维图谱法,对从竹节参中提取出的总皂苷类化合物进行定性分析。在波长为203 nm的检测条件下,以流动相水(0.5‰磷酸)和乙腈在流速为1.0 mL下梯度洗脱。利用该法,可鉴别竹节参总皂苷,方法简便、快捷。同时为竹节参总皂苷指纹图谱的建立提供充分依据。
TLC、UV and was applied to qualitative analysis of total saponin of Panax japonicus.By using acetonitrile-water（0.5‰H3PO4） as the mobile phase,with a flow rate of 1.0 mL· min-1,deterred at 203 nm,washed by gradient elution,the mian saponin can be separated in HPLC.The figure was applied to qualitative analysis,and to provide basis for its HPLC fingerprints.
出处
《武汉工业学院学报》
CAS
2012年第4期1-3,8,共4页
Journal of Wuhan Polytechnic University
