摘要
肝片形吸虫成熟虫体175条在常温下置不同培养液中体外培养,其结果是:在常温下,肝片形吸虫在适宜培养液中可存活10—12天,平均11天;在3类,17种培养液中以1640血清混合液为最佳,199血清混合液次之,台氏液最差,其中又以10％血清1640液为好;不同室温对肝片形吸虫的影响:在20～23℃与11～17℃的室温下,虫体产卵数与产卵天数均无明显差异,但在11～17℃下,虫体的存活时间明显延长。
One hundred seventy five mature worms of Fasciola hepatica were incubated under different conditions respectively, and the existing periods, the days and the amounts of egg production in worms incubated with various nutrient solusions at normal temperature were determined. The results indicated that: 1) when the mature Fasciola hepatica were incubated in an suitable nutrient solution at normal temperature, they were able to exist for 10 to 12 days(average, 11 days); 2)the nutrient solution made of RPMI 1640 mixed with serum was the best solution for incubating the mature worm of Fasciola hepatica, and the Tyroad's solution was inferior; 3)there were no significant differences in the days amounts of egg production between the worms incubated at 21 to 23℃ and the worms incubated at 11 to 17℃, but when the worms were incubated at a lower temperature (11 to 17℃), their existing periods were Ionger.
关键词
吸虫
肝片形
体外培养
常温
Fasciola hepatica, normal temperature,In vitro incubation.
