摘要
目的探讨雌激素受体(ER)和雄激素受体(AR)在肝癌患者癌组织和癌旁组织中的表达及意义。方法选择2007—2009年南通大学附属海安医院收治的肝癌患者45例,收集肿瘤切除后新鲜肝脏标本,按照自身配对法留取患者的癌组织和癌旁组织(距癌灶边缘5 cm)各45份。采用逆转录聚合酶链反应(RT-PCR)法检测ER和AR mRNA的表达,灰度比较法进行mRNA定量分析,以RT-PCR产物电泳进行定性分析;蛋白印迹法(WB法)检测ER和AR的表达,以相应的内参条带灰度值进行校正,获得相对蛋白表达量。结果 ER mRNA在肝癌患者癌组织中阳性表达37例,癌旁组织中阳性表达25例,差异有统计学意义(χ2=3.986,P<0.05);其阳性表达率在不同肿瘤分化程度、不同性别、浆膜侵犯与否、不同肿瘤直径、HBsAg阳性与否间比较,差异均有统计学意义(P<0.05);ARmRNA在肝癌患者癌组织中阳性表达38例,癌旁组织中阳性表达23例,差异有统计学意义(χ2=11.447,P<0.05);其阳性表达率在浆膜侵犯与否和不同肿瘤直径间比较,差异均有统计学意义(P<0.05)。ER mRNA在肝癌患者癌组织中的相对表达量为(0.2746±0.0828),癌旁组织中为(0.1891±0.0536),差异有统计学意义(t=5.8149,P<0.05);AR mRNA在肝癌患者癌组织中的相对表达量为(0.4642±0.2204),癌旁组织中为(0.1987±0.1719),差异有统计学意义(t=6.3720,P<0.05)。mRNA电泳显示,部分肝癌患者癌组织和癌旁组织同时表达ER或AR,部分仅在癌组织表达ER或AR,或仅在癌旁组织表达ER或AR。ER在肝癌患者癌组织中阳性表达41例,癌旁组织中阳性表达18例,差异有统计学意义(χ2=26.031,P<0.05);AR在肝癌患者癌组织中阳性表达43例,癌旁组织中阳性表达17例,差异有统计学意义(χ2=33.800,P<0.05)。ER在肝癌患者癌组织中的相对表达量为(0.68±0.02),癌旁组织中为(0.51±0.01),差异有统计学意义(t=8.84,P<0.05);其表达水平在不同性别、浆膜侵犯与否、HBsAg阳性与否�
Objective To investigate the expression and significance of estrogen receptors（ER）and androgen receptors（AR）in hepatocellular carcinoma and adjacent normal tissues.Methods Liver samples of 45 patients with hepatocellular carcinoma admitted to Haian Hospital affiliated to Nantong University from 2007 to 2009 were collected and 45 samples of carcinoma tissues and adjacent tissues（5 cm from carcinoma）were collected respectively.RT-PCR method was used to detect mRNA expression of ER and AR,fluorescence comparison method was used to quantitatively analyze mRNA,electrophoresis of RT-PCR products was performed to qualitatively analyze,WB method was used to detect protein expression of ER and AR.Results ER mRNA had positive expression in 37 cases of hepatocellular carcinoma tissues and 25 cases of adjacent tissues,and the difference was statistically significant（χ2=3.986,P0.05）.The positive expression rate of ER mRNA showed statistically significant differences in patients with difference carcinoma differentiation level,different sex,having serosal invasion or not,different carcinoma diameters and having positive HBsAg or not（P0.05）.AR mRNA had positive expression in 38 cases of hepatocellular carcinoma tissues and 23 cases of adjacent tissues,and the difference was statistically significant（χ2=11.447,P0.05）.The positive expression rate of AR mRNA showed statistically significant differences in patients with different serosal invasion status and different carcinoma diameters（P0.05）.The relative expression levels of ER mRNA in carcinoma tissues and adjacent tissues were（0.2746±0.0828）and（0.1891±0.0536）respectively,and the difference was statistically significant（t=5.8149,P0.05）.While the relative expression levels of AR mRNA in carcinoma tissues and adjacent tissues were（0.4642±0.2204）and（0.1987±0.1719）respectively,and the difference was statistically significant（t=6.3720,P0.05）.mRNA electrophoresis showed that some of the patients had ER and AR expression in both
出处
《中国全科医学》
CAS
CSCD
北大核心
2012年第33期3851-3855,共5页
Chinese General Practice
关键词
肝肿瘤
受体
雄激素
受体
雌激素
逆转录聚合酶链反应
印迹法
蛋白质
Liver neoplasms
Receptors
androgen
Receptors
estrogen
Reverse transcriptase polymerase chain reaction
Blotting
western
