摘要
目的:克隆获得与吴茱萸抗逆性密切相关的Cu/Zn-SOD基因cDNA全长序列。方法:采用RACE技术获得了该基因的开放阅读框(ORF)和3,5,端非编码区。结果:该基因全长717 bp,ORF区为459 bp,编码152个氨基酸,GenBank登录号为JQ285851。结论:该基因为吴茱萸Cu/Zn-SOD基因cDNA全长序列,与其它植物的Cu/Zn-SOD基因具有较高的同源性。
Objective ： To clone full-length sequence of Cu/Zn-SOD gene of Evodia rutaecarpa. Methods ： The genes ORF, 3, and 5, noneoding regions were achieved using the RACE technology. Results： This genes full-length was 717 bp, the open reading frame was 459 bp and 152 amino acids were encoded. The accession number of GenBank was JQ285851. Conclusion： This Cu/Zn-SOD gene is full-length cDNA sequence of Evodia rutaecarpa and it has high homology than other plants.
出处
《中药材》
CAS
CSCD
北大核心
2012年第9期1385-1388,共4页
Journal of Chinese Medicinal Materials
基金
国家"十二五"科技支撑计划项目"龙脑樟等6种大宗药材规范化种植基地及其SOP优化升级研究"(2011BAI04B04)
