摘要
该文建立了一种利用反相高效液相色谱法测定黄酒中草酸的方法，该方法以邻苯二胺为衍生剂，在高温酸性条件下与草酸反应，生成强紫外吸收化合物——2，3-二羟基喹喔啉，对其检测的最优色谱条件是：DionexAcclaim120C18柱（4．6×250mm，5μm）；以甲醇和0．1mol／L乙酸铵（15：85v／v）为流动相；流速1．2mL／min；检测波长314nm；柱温25℃。该方法测定黄酒样品中草酸的回收率为91．40%--103．70％，相对标准偏差小于9．30％。该方法简便、准确，适合测定黄酒中草酸的含量。
In this paper, a method of reverse phase high performance liquid chromatography （RP-HPLC） was developed to determine the oxalic acid content in Chinese rice wine. The derivatizing agent o-phenylenediamine was reacted with oxalic acid to form 2,3-dihydroxyquinoxalene, which had a strong UV absorption peak. The samples were separated on Dionex Acclaim 120 C18 column （4.6x250 mm, 5μm）, eluted with mixture of methanol and ammonium acetate 0.1 mol/L （15：85, v/v） at 1.2mL/min, and detected at 314nm, the column temperature was 25℃. The recovery of rice wine was 91.40% -103.70%, and the relative standard deviation （RSD） was less than 9.30%. This method was simple and accurate, can be used for the determi- nation of oxalic acid content in rice wine.
出处
《中国酿造》
CAS
2012年第11期159-162,共4页
China Brewing
基金
国家重点基础研究发展计划‘973项目’(2012CB720802)
关键词
反相高效液相色谱
黄酒
草酸
2
3-二羟基喹喔啉
reverse phase high performance liquid chromatography （RP- HPLC）
Chinese rice wine
oxalic acid
2,3-dihydroxyquinoxalene
