摘要
目的研究葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH细胞的体外细胞毒性。方法采用四甲基偶氮唑蓝(MTT)法检测葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH的细胞毒性;倒置显微镜观察细胞形态;流式细胞仪定量分析细胞周期。结果作用48 h后,葫芦素B以剂量依赖方式抑制SK-N-SH细胞的生长,0.143和0.716μmol.L-1葫芦素B固体脂质纳米粒对SK-N-SH细胞的抑制率分别为(34.9±4.6)%和(53.6±6.3)%,与对应浓度的葫芦B原料组的(13.2±2.3)%和(43.4±5.5)%相比较有显著性差异(P<0.05)。葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH细胞的IC50为0.508μmol.L-1,而葫芦素B原料为12.6μmol.L-1。细胞形态观察结果显示,与二甲基亚砜(DMSO)组相比较,葫芦素B原料及葫芦素B固体脂质纳米粒组的细胞密度下降,细胞体积缩小,大多数细胞形状发生改变。流式细胞仪检测结果显示,葫芦素B原料可将SK-N-SH细胞抑制在S期和G2/M期,而葫芦素B固体脂质纳米粒可把SK-N-SH抑制在G2/M期。结论葫芦素B固体脂质纳米粒与葫芦素B原料药既能抑制人神经母细胞瘤SK-N-SH细胞增殖,又能诱导其凋亡,显示出较强的细胞毒性,且前者毒性更强。
OBJECTIVE To evaluate the cytotoxicity of cucurbitacin B solid lipid nanoparticles （SLN） on human neuroblastoma SK-N-SH cell line in vitro. METHODS The SK-N-SH cell line was treated with cucurbitacin B SLN at various concentrations. Growth suppression was evaluated by MTT method; apoptosis related alterations in morphology were ascertained under light microscopy. Flow cytometry（FCM） was used to investigate the distribution of cell life. RESULTS Free cucurbitacin B and cucurbitacin B SLN inhibited the growth of SK-N-SH cells after 48 h treatment in a dose dependent manner, with ICs0values of 0. 508 and 12. 6μmol·L^-1, respectively. The apoptotic rates at concentrations of 0. 143 and 0. 716μmol·L^-1 were （34. 9± 4. 6）% and （53.6± 6. 3 ）%, respectively, all of which were significantly higher than that of free cucurbitacin B （ 13.2 ± 2. 3） % and（43.4± 5.5） % （P 〈 0. 05 ）, respectively. Under microscopy, the cells treated with free cucurbitacin B and cucurbitacin B SLN exhibited characteristics of apoptosis including decreased cell density of groups, reduced cell volume, and changed form of majority cell. Flow cytometry analysis suggested that free cucurbitacin B retarded the progression of cell cycle at G2/M and S phase, and cucurbitacin B SLN retarded the progression of cell cycle at G2/M phase. CONCLUSION Cucurbitacin B can not only inhibit the proliferation but also induce apoptosis of human neuroblastoma SK-N-SH cell line, demonsting strong cytotoxicity. Cytotoxicity of cucurbitacin B SLN is higher than that of free cucurb- itacin B.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第23期1894-1898,共5页
Chinese Pharmaceutical Journal
