摘要
以聚乙二醇-聚L-乳酸为载体材料,用高压静电纺丝法制得包载厚朴酚的纤维毡。扫描电镜和Photoshop 5.0软件测定结果表明得到了表面光滑、直径较均一的纤维;广角X-射线衍射结果表明载药纤维表面无药物结晶。采用HPLC法测定了载药纤维毡中厚朴酚在含或不含蛋白酶K的磷酸盐缓冲液中的累积释放率。结果表明,当介质中加入蛋白酶K(含量为2.5 g/ml)后,厚朴酚的释放速率即明显加快。酶浓度由2.5 g/ml升至10 g/ml时,厚朴酚释放速率继续增加,但增幅较小。介质中无蛋白酶K存在时,将释放曲线分段后用Higuchi方程拟合效果较好;有蛋白酶K存在时,药物释放曲线在平台期前呈现一级释放动力学形式。
Magnolol loaded fiber mats were prepared by high-voltage static electrospinning with polyethylene glycol-poly （L-lactic acid） as carrier. The observation of scanning electron microscope and the measurement results of the Photoshop 5.0 showed that the surface of the electrospun fiber was smooth and the diameter of fiber was homogeneous. The results of wide-angle X-ray diffraction test showed that there was no crystalline state on fibrous surface. The release behaviors of magnolol from the fiber mats in phosphate buffer with or without proteinase K were determined by HPLC. The results showed that the release rate was significantly increased in the proteinase K containing medium, even the enzyme concentration was 2.5 μg/ml. The release rate of magnolol continuously rose with the increasing of enzyme concentration （from 2.5 μg/ml to 10 μg/ml）, but the rate was smaller. The release curves of the samples in enzyme free medium were divided into several sections to fit Higuchi equation well, while the drug release behavior before the platform in the enzyme-containing medium fit the first order equation well.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2012年第12期991-997,共7页
Chinese Journal of Pharmaceuticals
关键词
电纺纤维
厚朴酚
静电纺丝法
聚乙二醇-聚乳酸
蛋白酶K
药物释放
electrospun fiber
magnolol
static electrospinning
polyethylene glycol-poly （L-lactic acid）
proteinase K
drug release
