摘要
目的探讨急进高原缺氧环境中腹泻大鼠血清内毒素(LPS)和肿瘤坏死因子α(TNF-α)水平的变化及与肝功能损害的相关性,观察谷氨酰胺对肠黏膜屏障的保护作用。方法将45只雄性Wistar大鼠分为高原缺氧组、高原腹泻组及谷氨酰胺(glutamine,GLN)治疗组,每组15只。大鼠在平原饲养1个月后于24小时内急运至海拔4 767m高原,建立高原缺氧模型,分别于到达高原后7天取门静脉血,比较各组间脂多糖(LPS)、肿瘤坏死因子α(TNF-α)和肝功能丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBil)的变化。结果与高原缺氧组比较,高原腹泻组LPS(0.224±0.035)μg/L和TNF-α(128.6±20.1)ng/L水平明显升高(P均<0.01);与高原腹泻组比较,GLN治疗组血浆LPS(0.185±0.035)μg/L和TNF-α(108.8±16.9)ng/L水平明显降低(P均<0.05);与高原缺氧组比较,高原腹泻组血血浆中ALT(68.4±20.4)U/L vs(49.5±18.9)U/L、AST(59.6±22.9)U/L vs(42.9±20.2)U/L、TBil(37.4±18.0)μmol/L vs(24.3±13.1)μmol/L均有明显升高(均P<0.01);高原腹泻组LPS升高与ALT、AST、TBil水平呈显著正相关(均P<0.05)。结论高原缺氧环境中,腹泻可加重肠黏膜屏障破坏,可导致肠源性内毒素血症,吸收入血的内毒素可能参与高原缺氧环境肝功能损害过程,GLN治疗能够减轻小肠黏膜屏障损害,维持肠道正常功能。
Objective To investigate the correlations between the level of serum endotoxin（LPS） and tumor necrosis factor-α（TNF-α） as well as the changes of liver functions of rats with diarrhea in simulated acute high altitude hypoxia,and the protective efficacy of glutamine（GLN） on their intestinal mucosal barrier. Methods 45 male Wistar rats were equally divided into high altitude hypoxia group, high altitude diarrhea group,and GLN treatment group. The rats of the above groups were moved to an altitude of 4 767 meters within 24 h after being fed on the plain for 1 month, and the model of high altitude hypoxia was established. The portal vein blood samples of all rats were taken on day 7 after arrival at the above altitude, and their blood LPS, TNF-α, ALT, AST, and TBil were detected. Results When compared with those of the high altitude hypoxia group, the levels of LPS（0. 224± 0. 035）μg/L, TNF-α（ 128.6 ± 20.1 ） ng/L,ALT（68.4±20. 4） U/L vs （49.5±18.9） U/L, AST（59.6±22.9） U/L vs （42.9±20.2） U/L,and TBil（37.4± 18.0） btmol/L vs （24.3±13.1） μmol/L were significantly higher （all P d0.01） ,and the level of LPS was positively correlated with the level of ALT,AST,or TBil（ P d0.05） in high altitude diarrhea group. When compared with the high altitude diarrhea group, the levels of LPS （0. 185 ± 0.035） μg/L and TNF-α （108.8±16.9） ng/L were significantly lower in GLN treatment group （all P 〈0.05）. Conclusion In high altitude hypoxia environment,diarrhea may increase the damage of intestinal mucosal barrier and lead to intestinal endotoxemia, and the LPS absorbed into the bloodstream may be involved in the process of liver damage. GLN treatment can extenuate the damage of intestinal mucosa and maintain the normal intestinal functions.
出处
《临床荟萃》
CAS
2012年第23期2061-2063,共3页
Clinical Focus
基金
全军"十一五"课题项目(06MA089)
关键词
高原病
肠黏膜
内毒素类
肿瘤坏死因子Α
肝功能不全
谷氨酰胺
altitude disease
intestinal mucosa
endotoxinsltumor necrosis factor-α
hepatic dysfunction
glutamine
