摘要
用ESI-MS研究了一系列结构具有可比性的N-磷酰化多巴胺与牛胰岛素的非共价相互作用,比较了磷上不同烷氧取代基对相互作用影响。结果表明烷氧取代基上烷基碳原子个数和其排列顺序对二者相互作用有较大影响。取代基上碳链越长溶液中胰岛素的构象越趋于收缩,易于形成高计量比复合物;而支链或直链取代对胰岛素构象的影响程度差别不大。磷酰化多巴胺与胰岛素形成复合物的稳定性随着取代基的增长而增强;直链取代的磷酰化多巴胺与胰岛素形成的复合物比支链取代的磷酰化多巴胺与其形成的复合物稳定。这一结论对设计合成新型多巴胺类化合物有一定的指导意义。
ESI-MS is an effective way to study intermolecular interaction. In this paper, the non-covalent interaction between a series of synthesized N-phosphoryl dopamine and insulin was studied by using ESI-MS. The effects of different groups of P on N-phosphoryl dopamine were compared. The results showed that length and sequence of alkyl group of alkoxyl group on P were very important for the interaction under ESI-MS conditions. N-phosphoryl dopamines with longer carbon chains could contract the protein configuration more greatly and form complex with higher stoichiometric proportion in solution, while the sequence of carbon chain with a same number carbon atom have no obvious difference on contraction of protein. The result also showed that the length of carbon chain also had an obvious effect on the stability of complexes. Straight chain was more beneficial for the stability than branched chain when the number of carbon was equal. This conclusion was valuable for design of new active dopamine molecules.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第6期73-77,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(21004055
20672104)
