摘要
目的:探讨Treg、Th17细胞及Treg/Th17比率与肝功能的相关性,以便更好地反映慢性乙型肝炎(CHB)患者肝脏免疫炎症。方法:收集41例HBeAg阳性CHB患者、29例HBeAg阴性CHB患者和20例健康志愿者的外周血,采用流式细胞术检测Treg细胞(CD4+CD25highCD127low)和Th17细胞(CD3+CD8-IL-17+)的频数;采用酶联免疫吸附试验(ELISA)检测血清TGF-β1水平;采用全自动生化仪检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBil)、直接胆红素(DBil)等指标。结果:CHB患者Treg和Th17细胞的频数明显高于健康对照组(P<0.05),Treg/Th17比率低于健康对照组(P<0.05)。CHB患者Treg细胞频数与TBil、DBil和AST呈正相关(P<0.05),Th17细胞与以上指标均无相关性(P>0.05)。HBeAg阳性CHB患者Treg/Th17比率与TBil、DBil和ALT呈正相关(P<0.05),且随病情进展Treg/Th17比率呈上升趋势(P>0.05),TGF-β1则明显升高(P<0.05);HBeAg阴性患者Treg/Th17比率与ALT呈负相关(P<0.05)。综合治疗平均2个月后,HBeAg阳性患者Treg/Th17比率仍与TBil保持正相关(P<0.05)。结论:Treg细胞和Treg/Th17比率与CHB患者的肝功能具有良好相关性。监测Treg、Th17细胞及其比率,有助于反映CHB患者肝组织的免疫炎症。
Objective：To reflect the immune inflammation of the liver tissue of patients with chronic hepatitis B（CHB） by explore the correlation of Treg/Th17 ratios and liver function indicators.Methods：We collected the peripheral blood from 41 cases of HBeAg-positive CHB patients,29 cases of HBeAg negative CHB patients and 20 healthy volunteers.The frequencies of Treg（CD4＋CD25highCD127low） and Th17（CD3＋CD8-IL-17＋） cells in peripheral blood were assessed by flow cytometric analysis.The plasma TGF-β1 was measured by enzyme-linked immunosorbent assay（ELISA）.The serum Alanine aminotransferase（ALT）,Aspartate aminotransferase（AST）,Total bilirubin（TBil）,Direct bilirubin（DBil） and other liver function indicators were detected by automatic biochemical analyzer.Results：The frequencies of Treg and Th17 cells in CHB group was significantly higher than the healthy control group（P0.05）,while the ratios of Treg/Th17 was lower than the healthy control group（P0.05）.The frequencies of Treg cells were positively correlated with TBil,DBil and AST in patients with chronic hepatitis B（P0.05）,while Th17 cells was not correlated with above indicators（P0.05）.The ratios of Treg/Th17 were positively correlated with TBil,DBil and ALT showed rising trend with the progress of the disease in HBeAg-positive CHB patients.The plasma TGF-β1 was significantly increased with the progress of the disease（P0.05）.While the ratios of Treg/Th17 were negatively correlated with ALT in HBeAg-negative CHB patients（P0.05）.The ratios of Treg/Th17 remained positive correlation with TBil after an average of two months of the comprehensive treatment in HBeAg-positive patients（P0.05）.Conclusion：The frequencies of Treg cells and the ratios of Treg/Th17 were well correlative to liver function indicators in patients with chronic hepatitis B,and the ratios of Treg/Th17 were correlated with the severity of HBeAg-positive CHB patients.Monitoring the ratios of Treg/Th17 can help reflect the immune inflammat
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第11期1036-1040,共5页
Chinese Journal of Immunology
基金
福建省医学创新课题(2012-CX-33)
泉州市科技计划项目资助课题(2011Z63)
泉州市优秀人才培养专项经费(10A07)资助
