摘要
目的:研究中药预防流感的作用与黏膜免疫相关性。方法:(1)不同预防方药预防给药对正常小鼠黏膜免疫的影响:将BALB/c小鼠随机分6组。设空白对照组(生理盐水)、利巴韦林组、银翘散制剂、玉屏风散制剂、冰香散滴鼻组、冰香散雾化组。给药第12天,杀剖小鼠,空白对照组称体重和肺重测定肺指数。各组均采血分离血清,收集鼻咽冲洗液、支气管肺泡灌洗液、肠道冲洗液进行ELISA法检测IgA含量。(2)不同预防方药预防给药对流感病毒感染小鼠黏膜免疫的影响:将BALB/c小鼠随机分6组,设病毒对照组及药物试验组(同前)。试验药物在滴鼻感染15 LD50流感病毒液感染攻击前提前给药一周,攻击后继续给药5天。共给药12天,每天一次给药。感染后5天,杀剖小鼠,称体重和肺重测定肺指数。采血分离血清,收集鼻咽冲洗液、支气管肺泡灌洗液、肠道冲洗液进行ELISA法检测IgA含量。结果:(1)不同预防方药预防给药对正常小鼠黏膜免疫的影响:连续给予正常小鼠12天试验药物,利巴韦林、银翘散制剂、玉屏风散制剂、冰香散对流感病毒鼠肺指数均有显著的改善作用,肺指数抑制率达到25.46%～41.61%。与空白对照组相比,银翘散制剂口服与冰香散雾化给药血清IgA水平显著提高;冰香散滴鼻给药鼻咽部sIgA水平显著提高;利巴韦林组给药支气管-肺泡sIgA水平下降;银翘散、玉屏风散、冰香散给药肠道sIgA水平有一定的提升作用,其中玉屏风散制剂作用明显。(2)不同预防方药预防给药对流感病毒感染小鼠黏膜免疫的影响:病毒感染小鼠攻击前预先连续给药7天,病毒感染第5天,与病毒对照组相比,利巴韦林给药血清IgA水平显著降低、冰香散雾化给药血清IgA水平显著提高;冰香散滴鼻给药能使鼻咽部sIgA水平显著提高;利巴韦林组给药支气管-肺泡sIgA水平下降,玉屏风散与冰香散雾化给药支气管-肺泡sIgA
Objective：To investigate the positively relations between the efficacy of Chinese Medicine in the prevention influenza and mucosal immune.Methods：（1） Effect of several different preventive herbs on mucosal immunity in normal mice： Normal BALB/c mice were divided into different groups randomly,Blank control group,Ribavirin group（Oral administration,100 mg/kg）,Yinqiao Powder Preparation,Jade Screen Powder Preparation,Bing-xiang Powder Preparation（Nebulised inhalation and Nasal drops）.All trial drug groups were administrated to mice 12 d.On the 12th day,mice were measured to collect the sera,rinse solution of intestimal,nasopharynx,lung and the IgA antibodies in them were detected by ELISA,and Blank control group weighted the body and lung to measure the pulmonary index.（2） Effect of several different preventive herbs on mucosal immunity in mice infected with influenza virus： BALB/c mice infected with influenza were divided into six groups randomly,Virus control group,Ribavirin group,Yinqiao Powder Preparation,Jade Screen Powder Preparation,Bing-xiang Powder Preparation（Nebulised inhalation and Nasal drops）.All trial drug groups were administrated to mice 7 d before infection.On the 5th day post-infection,mice were measured to collect the sera,rinse solution of intestimal,nasopharynx,lung and the IgA antibodies in them were detected by ELISA,and weighted the body and lung to measure the pulmonary index.Results：（1） Effect of several different preventive herbs on mucosal immunity in normal mice： Pulmonary-index and virus titer decreased significantly（P0.01） in these groups of Ribavirin,Yinqiao Powder Preparation,Jade Screen Powder Preparation and Bing-xiang Powder Preparation by administration 7 d before infection,inhibition ratios of pulmonary-index were 25.46% up to 41.61%.Compared with the Blank control group,the level of sera IgA in the Yinqiao Powder Preparation group and Bing-xiang Powder Preparation by nebulised inhalation were significantly increased after the treatment.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第11期992-998,共7页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81072948)
广东省建设中医药强省立项科研课题(2010397)资助
关键词
中药
预防流感
黏膜免疫
免疫球蛋白A
Chinese Medicine
Preventing influenza
Mucosal immunity
Immunoglobulin A
