摘要
为观察大鼠中枢神经系统中与心血管反射性调节相关的部位 ,用硝普钠静脉注射制作大鼠低血压模型 ,以抗 FOS蛋白免疫组织化学方法 ,观察全脑 FOS阳性神经元分布。 FOS阳性细胞在下列区域集中分布 :延髓内脏带、脑桥臂旁外侧核、蓝斑、A5区、中脑中央灰质腹外侧区 ,下丘脑室旁核、视上核、视交叉上核数量更多。此外亦见于丘脑室旁核、缰内侧核、杏仁核、终纹床核、隔外侧核和皮质的额皮质 1和 2区、扣带皮质及梨状前皮质。免疫组化染色对照 :从每一组切片中取一定数量的切片 ,0 .1 mol/L PBS代替兔抗 FOS抗血清进行反应 ,各步骤同前。其结果未见 FOS阳性结构。本研究表明 ,中枢神经系统内从皮质至延髓存在不同的血压调控区 ,它们互相联系形成一复杂的中枢调控网络。
To observe the areas in the central nervous system of rat in which the cardiovascular functions are adjusted reflexly, animal models of hypotention in rats were induced by injecting sodium nitroprusside into the vein. And the distributions of FOS positive neurons in the brain were studied immunohistochemically. Hypotention induced a significant increase in FOS immunoreactivity in the medullary visceral zone, parabrachial nucleus, locus coeruleus, A5 area, ventrolateral area of periaqueductal gray. FOS positive neurons expressed highly in the paraventricular nucleus, supraoptic nucleus, suprachiasmatic nucleus of hypothalamus too. Dense FOS immunoreactive staining were also seen in the paraventricular nucleus of thalamus, lateral habenular nucleus, central nucleus of amygdala, bed nucleus of stria terminalis, lateral septal nucleus, frontal cortex, cingulate gyrus and prepiriform cortex. The results show that several blood pressure modulating areas exist from cerebral cortex to medulla, and they connect each other forming a complicated central modulating network.[
关键词
延髓内脏带
中枢神经系统
心血管调节
FOS
medullary visceral zone
CNS
cardiovascular modulation
FOS immunohistochemistry
rat
