摘要
运用免疫组化ABC法探讨了脊髓挤压伤后不同时间腹角NT - 3表达的变化 .结果 :对照组可见NT - 3免疫反应阳性细胞分布于腹角的大神经元和少量小神经元 ,胞核、胞浆均染色 .此外 ,亦见少量阳性胶质细胞 .脊髓挤压伤后 2 4h ,神经元内NT - 3免疫反应强度较正常时为深 (P <0 0 5) .挤压后 7d ,NT- 3免疫反应强度及阳性细胞数目均较正常及 2 4h组者有显著增加 (P <0 0 5) .提示 ,NT - 3不仅可能参与了正常脊髓腹角神经元的生理过程 ,而且可能在脊髓损伤修复中发挥作用 .
WTBZ In this study,ABC method of immunohistochemistry was used to explore the changes of expression for NT-3 in neurons of ventral horn in different time after crushed spinal cord injury(cSCI). The results showed that NT-3 positive neurons were distributed in neurons of ventral horn in intact group and NT-3 immunorecative degree increase greatly than that of contral group at 24 h following injury( P <0 05).Comperetively,in 7 d group,not only NT-3 immunoreactive degree,but also NT-3 positive neuronal numbers apperently increase than that of contral group and 24 h group following injury ( P <0 05).The results indicated that the expression of NT-3 involves in the physiological function of spinal cord in adult rat and may play an important role in the repair of nerves following cSCI.
出处
《昆明医学院学报》
2000年第1期60-62,共3页
Journal of Kunming Medical College
基金
云南省自然科学基金!99C0 0 0 6R
