摘要
[Objective]The paper was to screen the plant extracts with strong antifungal activity. [Method] With R. solani as the test object, the antifungal activities of 30 kinds of plant extracts were carried out primary screening by mycelial growth rate method. [Result] Twelve kinds of plant extracts including Pinus yunnanensis (leaf), Phryma leptostachya (stem and leaf), Pueraria peduncularis (root, stem and leaf), Pinus armandiifruit (fruit), Aconitum brachypodum (stem and leaf), Phellodendron amurense (stem and leaf), Rhaponticum uniflorum (root), Alnus cremastogyne (leaf), Rehmannia glutinosa (root), Cryptomeria fortunei (stem and leaf) and Berberis pruinosa (stem and leaf) had strong activities against R. solani with growth inhibition rates over 80% as the concentration was 10 mg/ml,among which the extracts of P. yunnanensis (leaf), P. leptostachya (stem and leaf), P. peduncularis (root, stem and leaf) and P. armandiifruit (fruit) showed the inhibition rate above 95%, significantly higher than other plant extracts. The secondary screening results of antifungal activities of the above 12 plant extracts were consistent with the primary screening. [Conclusion] The study laid foundation for further development of high efficient, safe and environmental-friendly new fungicides.
[Objective]The paper was to screen the plant extracts with strong antifungal activity. [Method] With R. solani as the test object, the antifungal activities of 30 kinds of plant extracts were carried out primary screening by mycelial growth rate method. [Result] Twelve kinds of plant extracts including Pinus yunnanensis (leaf), Phryma leptostachya (stem and leaf), Pueraria peduncularis (root, stem and leaf), Pinus armandiifruit (fruit), Aconitum brachypodum (stem and leaf), Phellodendron amurense (stem and leaf), Rhaponticum uniflorum (root), Alnus cremastogyne (leaf), Rehmannia glutinosa (root), Cryptomeria fortunei (stem and leaf) and Berberis pruinosa (stem and leaf) had strong activities against R. solani with growth inhibition rates over 80% as the concentration was 10 mg/ml,among which the extracts of P. yunnanensis (leaf), P. leptostachya (stem and leaf), P. peduncularis (root, stem and leaf) and P. armandiifruit (fruit) showed the inhibition rate above 95%, significantly higher than other plant extracts. The secondary screening results of antifungal activities of the above 12 plant extracts were consistent with the primary screening. [Conclusion] The study laid foundation for further development of high efficient, safe and environmental-friendly new fungicides.
基金
Supported by Youth Fund Project of Yunnan Agricultural University