摘要
目的:研究五味子超临界萃取物对β淀粉样蛋白(Aβ25-35)所致的PC12细胞损伤后生存状态和活力的保护作用。方法:体外培养PC12细胞,建立Aβ引起的细胞损伤的AD细胞模型,采用MTT法和LDH法检测细胞生存活力。结果:五味子超临界萃取物能降低LDH含量,升高细胞线粒体脱氢酶的活性,增加活细胞数。结论:五味子超临界萃取物能减轻Aβ25-35损伤后的PC12细胞的受损程度,保护细胞膜,提高细胞生存活力。
Objective ： To study the protective effects of supercritical extraction obtained from Schisandra chinensis ior the survival condition and vigor of PC12 cells damaged by Aβ 25 - 35. Methods ： The PC cells were cultured in vitro. An AD cell model about cell damage caused by Aβ was established. The survival condition and vigor of PC12 cells were de- tected by using the MTT and LDH methods. Results： Supercritical extraction of Schisandra Chinensis could reduce the concentration of LDH and increased the activity of mitochondria dehydrogenase of PC12 cells. The amount of living PC12 cells increased. Conclusion： The extent of injury of Aβ 25 -35 damaged PC12 cells can be relieved and the cell mem- brane can also be protected by using supercritical extraction of Schisandra Chinensis. The extraction is good for impro- ving the survival condition and vigor of PC12 cells.
出处
《中医药学报》
CAS
2012年第5期27-29,共3页
Acta Chinese Medicine and Pharmacology
基金
国家自然科学基金资助项目(30472128)
黑龙江省自然科学基金资助项目(D201014)
