摘要
对近江牡蛎体内异养细菌进行1周年的监测(2010年1月～2010年12月),分离得到的异养菌(180株)鉴定到属,并检测产生蛋白酶、淀粉酶和脂肪酶的能力。结果显示,正常贝体内异养菌和弧菌数分别为7.3×103～6.6×104CFU/g和8.0×10～8.2×103CFU/g,随机分离的菌株分属肠杆菌科的部分属Enterbacteriaceae、弧菌属Vibrio、气单胞菌属Aeromonas、假单胞菌属Pseudomonas、葡萄球菌属Staphylococcus、发光杆菌属Photobacterium、无色杆菌属Achromobacter、芽孢杆菌属Bacillus等。产酶试验结果表明,正常贝体分泌蛋白酶和纤维素酶的菌株数量最高出现于9月份,产酶菌株比例分别高达91.7%和63.9%,分泌淀粉酶和脂肪酶菌株数量高峰出现于7月份,产酶菌株比例均达81.8%,其中15株能同时分泌这4种酶。
The quantitative and qualitative studies on the bacterial population in farmed oyster Crassostrea hongkongensis were surveyed for one year. The strains （about 180） from the body of oyster were purified and identified for their genus, and the enzyme-producing （ protease, amylase, lipase and cellulase ） of the strains was measured. The results showed that the the heterotrophic bacteria and vibrio were 7.3 × 10^3 - 6.6 × 10^4 CFU/g and 8.0 × 10-8.2×10^3 CFU/g in heathy oysters,respectively. The 180 strains were identified as Enterbacteriaceae , Vibrio ,Aeromonas , Pseuclomonas , Staphylococcus, Photobacterium , Achromobacte , Bacillus and so on. The zymogenic experiment results showed that the crest value of strains producing protease and cellulose appeared in Sep. , and the percentage reached 91.7 % and 63.9%, respectively, while the peak of strains producing amylase and lipase emerged in Jul. , and the percentage reached 81.8%. The results also showed that there were 15 strains produced the 4 enzymes simultaneously.
出处
《海洋环境科学》
CAS
CSCD
北大核心
2012年第6期851-854,866,共5页
Marine Environmental Science
基金
现代农业产业技术体系建设专项资金-贝类产业技术体系科学家岗位专项经费(CARS-48)
广东省农业攻关项目-贝类产业推进关键技术研究与示范(2010B20201014)
关键词
近江牡蛎
异养菌
弧菌
消化酶
Crassostrea hongkongensis
heterotrophic bacteria
vibrio
digestive enzyme
