摘要
应用cDNA-SRAP标记方法,筛选出19对引物,对8-12月所采集的24个蜡梅花芽(花朵)样品所制备的cDNA模板进行扩增,研究蜡梅花发育各时期的基因表达情况,并对部分差异片段进行了表达分析和功能预测。结果表明,cDNA-SRAP分子标记技术操作简便,能产生较丰富的条带,是进行差异显示分析研究的有效途径;与玉米淀粉去分支酶基因、欧洲山杨纤维素合成酶基因、玉米抗锈病基因和小麦多酚氧化酶基因相似性高的差异表达片段,可能对蜡梅花发育和抗性表现起着重要的作用。
Nineteen pairs of cDNA-SRAP primers were designed to amplify cDNA samples of 24 Chimonanthus praecox flower buds/tepals representing different developmental stages.The biological functions of a portion of differentially expressed genes were predicted and characterized.The results showed that cDNA-SRAP methods is simple,cheap,effective in generating relatively abundant bands and a valid approach to study differentially expressed genes.It indicated that fragments having high sequence similarity with Zea mays Zpu1,Populus tremuloides CesA4,Zea mays RP3-1 and Triticum aestivum PPO may play an important role in flower development and resistance to stress.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2012年第6期693-698,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30800762
30972019)
关键词
蜡梅
cDNA-SRAP
差异显示
基因表达
发育阶段
Chimonanthus praecox
cDNA-SRAP
differential display
gene expression
developmental stage
