摘要
为进一步研究常夏石竹愈伤组织的再分化,采用L9(34)正交设计研究了不同外植体、不同植物生长调节物质及其配比对常夏石竹愈伤组织诱导和生长的影响。结果表明:外植体是影响常夏石竹诱导率的最主要因素,茎段愈伤组织诱导率最高达88.5%,且发生最早,愈伤化程度高;茎段是诱导愈伤组织最佳外植体;最有利于常夏石竹愈伤组织诱导的植物生长调节剂组合为MS+6-BA 1.5 mg/L+NAA0.5mg/L+蔗糖30g/L+琼脂7g/L+PVP 0.1%。
To study on the redifferentiation of D.plumarius calli in further,the effects of different explants and plant growth regulating substances and their matching on induction and growth of D.plumarius calli were studied by L9（34）orthogonal design.The results showed that the main factor which mostly influenced the inductivity was explants.the stem had the highest induction ratio（88.5%）,and its occurrence was the earliest.The plant growth regulator combination of MS＋6-BA 1.5 mg/L＋NAA 0.5 mg/L＋30 g/L sucrose ＋7 g/L agar＋PVP 0.1% was the most propitious to the induction of calli.
出处
《贵州农业科学》
CAS
北大核心
2012年第10期1-2,6,共3页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目"斑马鱼母源性补体的传递和功能"(31000410)
陕西省教育厅项目"猕猴桃Ac/Ds插入突变体库的构建"(12JK0832)
陕西省教育厅项目"Ac/Ds转座元件转化猕猴桃的研究"(09JK434)
关键词
常夏石竹
外植体
组织培养
诱导
Dianthus plumarius
explants
tissue culture
induction
