摘要
以转TaEBP基因小麦新品系G258及其受体亲本衡观35种子为材料,研究了20%PEG人工模拟干旱胁迫对参试材料种子萌发期的胚芽鞘长度、胚根数、主胚根长度的影响。在此基础上,结合盆栽控水法研究了水分胁迫对转基因小麦新品系生长发育中后期抗旱相关生理生化及形态指标的影响。结果显示:在20%PEG模拟干旱胁迫下,参试材料的胚芽鞘长、胚根数、主胚根都有所降低,而G258降幅较小,并且在同一胁迫下胚芽鞘长度、胚根数、主胚根指标都优于HG35;盆栽控水条件下,随着水分胁迫加剧和生长发育的推进,参试材料的水分利用率呈下降趋势,而转基因小麦G258下降幅度较小。丙二醛含量呈上升趋势,对照品种HG35上升幅度更大,G258上升幅度较小;在中度和重度胁迫下,转基因小麦G258每一生育期脯氨酸、ABA含量都明显高于对照品种HG35。上述结果表明,转基因小麦新品系G258具有较强的抗旱节水性能。
Using new transgenic wheat line＇G258＇with TaEBP gene and its receptor parent＇HG35＇as materials,we studied the effects of 20% poluethylene glycol（PEG）-simulated drought stress on the coleoptile length,radicle length and main radicle numbers of transgenic wheat at geminating period.Then a pot experiment was conducted under different water stress to study the effect of drought stress on the physiological and biochemical,morphological indexes of transgenic wheat during the middle and later growing stage.The result indicated that the coleoptile length,radicle length and main radicle numbers of wheat seedlings reduced under 20% PEG simulated drought stress,but the declining range of the transgenic line＇G258＇was smaller,and the coleoptile length,radicle length and main radicle numbers of its seedlings were obviously higher than that of＇HG35＇ under the same stress condition.Under potted condition,the water use efficiency showed downtrend with the increasing of drought stress,but that of the transgenic wheat line ＇G258＇ declined more slowly.MDA content showed uptrend,with the corresponding contrast＇HG35＇rose more steeply,and the transgenic line ＇G258＇ rose more slowly.Under moderate and serious stress conditions,the proline content and ABA content of transgenic wheat line ＇G258＇ were significantly higher than that of the contrast ＇HG35＇ during whole growth period.These results showed that the new transgenic wheat line＇G258＇ had higher performance on drought resistance and water-saving.
出处
《干旱区资源与环境》
CSSCI
CSCD
北大核心
2012年第12期167-171,共5页
Journal of Arid Land Resources and Environment
基金
国家转基因生物新品种培育科技重大专项(No.2009ZX08002-008B)资助
