摘要
在1-脱氧野尻霉素的高效液相色谱法分析中,利用响应面分析法优化了荧光衍生化的最佳条件。根据Box-Behnken中心组合实验设计原理,在单因素实验基础上,以1-脱氧野尻霉素回收率为响应值,应用响应面分析法研究衍生化反应时间、反应温度和衍生化试剂用量各因素间的交互作用及对1-脱氧野尻霉素回收率的影响。确定最佳衍生化条件为:衍生化反应时间22min、衍生化反应温度29℃、衍生化试剂用量0.43mmol/L,实际回收率为99.4%。
For the purpose of establishing a high performance liquid chromatography method for the determination of 1-deoxynojirimycin（1-DNJ）,the optimal derivatization conditions were studied by response surface methodology. Based on the result of single factor experiments,the Box-Behnken design was used to investigate the effect of derivative time,temperature and the amount of derivative reagent on the recovery of 1-deoxynojirimycin. The derivative conditions were found to be 22min,29℃ and 0.43mmol/L and the recovery was 99.4%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第22期63-67,共5页
Science and Technology of Food Industry
基金
教育部科学技术研究重点项目(211003)
北京市教育委员会科技发展计划面上项目(KM201110017002)
