摘要
应用PCR-DGGE方法分别对天目山和玲珑山的4种典型森林土壤细菌群落结构进行了研究分析。结果表明,阔叶林、杉木林、马尾松林和毛竹林在天目山土壤中的DGGE条带数分别为49、45、51和51条,在玲珑山土壤中的DGGE条带数分别为42、42、44和48条。不同森林植被土壤DGGE带谱差异不明显,天目山和玲珑山4种森林土壤分别有31条和23条共性条带,分别占总数的61%和48%以上,然而每种林分也有各自的特征条带。相对而言,两个样地杉木林与其他3种林分的土壤细菌群落结构相似度较低,相似值分别为0.72和0.68,其土壤细菌多样性指数也明显低于其他3种林分,而毛竹林和马尾松林多样性指数则没有显著差异。同时分析两个采样区的土壤细菌DGGE发现,土壤性质对细菌群落结构的影响大于植被。
Broad-leaved forest, Chinese fir forest, Mason pine forest and Bamboo grove are widely distributed in subtropical areas of South China. To investigate more information of soil bacterial community structures of natural forests, PCR-DGGE technology was applied to study structures of the soil bacterial communities in these four types of natural forests distributed in from the Tianmu Mountains and Linglong Mountains. Results show that the number of DGGE strips detected in the four kinds of forest soils in the Tianmu Mountains was 49, 45, 51 and 51 , and in the Linglong Mountains, it was 42, 42, 44 and 48, respectively. DGGE analysis reveals little variation between the soils in fingerprint. The four soils in the Tianmu Mountains had 31 DGGE strips in common, while the soils in the Linglong Mountains had 23, accounting for 61% and 48% or more, respectively, of the total strips detected, and what is more, each type of forest had its own characteristic strips;Relatively speaking, the two sample lots of Chinese fir forest were both quite low in similarity to the other sample lots of other forest stands in soil bacterial structure, being 0.72 and 0.68 respectively in value, and diversity indices of their soil bacterial communities were obviously lower than the others＇. However, Bamboo groves and Mason pine forests were not much different in soil bacteria diversity. DGGE analysis of soil bacteria from two sampling sites revealed that the effect of soil properties was greater than that of vegetation on soil bacterial community structure.
出处
《土壤学报》
CAS
CSCD
北大核心
2012年第6期1185-1193,共9页
Acta Pedologica Sinica
基金
浙江省自然科学基金项目(Y3100578)资助
