摘要
目的监测本地区2002—2011年间与腹泻有关的病原菌群10年的变化和耐药趋势,为流行病学研究、疫苗的制备及临床合理用药提供依据。方法通过收集腹泻患者的大便进行SS培养,阳性致病菌经生化及血清学进一步鉴定到属和种,并以纸片扩散法测定了12种抗菌药物的敏感性。结果 10年分离到腹泻病原菌2352株,主要包括沙门菌、志贺菌、弧菌、气单胞菌、类志贺毗邻单胞菌及致泻大肠埃希菌。前5年志贺菌最多占52.90%,有逐渐减少的趋势,2007年以后减少至32.80%;而沙门菌与致泻大肠埃希菌分别由4.90%和3.60%增至15.40%和19.70%。不同菌属对抗生素的耐药率有差别。腹泻病原菌对氨苄青霉素,复方磺胺甲噁唑的耐药率分别达到75%~85%和51%~66%,且对广谱青霉素类、氟喹诺酮类和头孢菌素类的耐药率在10年间明显升高,多重耐药率由24.67%上升到29.01%。结论本地区引起腹泻的病原菌属种的种类多,对不同抗生素的耐药有差异,耐药及多重耐药菌明显增加,应重视监测并指导防治。
Objective To monitor the constitutes and resistant tendency of diarrhea bacteria genus in diarrheal patients in our hospital from 2002 to 2011 to offer the date for guiding epidemiologic study, vaccination research and clinical treatment. Methods We had cultured by SS agar and identified diarrhea bacteria to group and species with the biochemical and serologic test. Then, we tested the susceptibility of diarrhea bacteria to 12 kinds antimicrobial agents. Results 2,352 isolates including Salmonella, Shigella, Vibrio, Aeromonas, diarrheagenic Escherichia coll. caused diarrhea were identified during 10 years. Among them, Shigella was the most and occupied 52.90 and reduced gradually to 32.80% in later five years. Shigella had reduced to 32.80% and Salmonella and Escheriehia coli diarrheogenic had increased from 4.90% and 3~0% to 15.40% and 19.70% respectively. The higher resistant rate of diarrhea bacteria happen to ampicillin (75%-85%) and trimethoprim-sulfamethoxazole (51%-66%). The resistant rate of diarrhea bacteria to broad-spectrum penicillins, fluoroquinolones and cephalosporins was increased obviously during 10 years. Furthermore, multiple antibiotic resistant strains was increased from 24.67% to 29.01%. Conclusion There was many kinds of diarrhea bacterial caused diarrhea in local area and different drug resistant rate to different antibiotics. Resistant and Multiple resistant-drug bacteria was increasing during research time. It is necessary to supervise for prevention and treatment.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2012年第11期856-860,共5页
Chinese Journal of Antibiotics
基金
军队十二五重点课题BWS11C073
关键词
腹泻病原菌
耐药趋势
组成特征
Diarrhea bacteria
Antimicrobial resistant tendency
Distribution characterization