摘要
【目的】为了明晰柿属植物种间亲缘关系和探究逆转座子与该属植物倍性进化的相关性,【方法】以16种24份柿属植物为试材,利用IRAP(Inter-Retrotransposon Amplified Polymorphism)技术和UPGMA聚类分析。【结果】采自我国南热带的供试基因型独自成组,与其采集地域相吻合,反映了它们较古老的分类学地位;采自我国北亚热带和温带的基因型独立于南亚热带基因型组,含丰富的染色体倍性,且相互间混聚在一起,无明显地域趋同性,反映了该区域基因型间广泛的基因交流和适应性进化;从UPGMA聚类图反映的不同区域柿属基因型间的相对位置来看,逆转座子活性与其倍性进化及生态适应性间存在明显的相关性。【结论】采自我国南亚热带的柿属植物可能更加古老;逆转座子在柿属种间倍性进化过程中可能发挥了一定的作用。
【Objective】The objective of the present study is not only to elucidate inter-species genetic relationships but also to explore the relations between retrotransposon activity and ploidy evolution in the genus of Diospyros.【Method】16 species including 24 genotypes were examined using both IRAP（Inter-Retrotransposon Amplified Polymorphism） and UPGMA cluster method.【Result】The genotypes collected from south subtropical regions of China were grouped independently,in consistent with their collected geographical position,which reflected their ancient taxonomy status;Those collected from both north subtropical and temperate regions of China were gathered together but apart from those from south subtropical regions.The second above mentioned group contained abundant ploidy and crossing clustered from different region,no obvious trends of geography,which displayed widely genetic exchanges and adaptive evolution among them;In view of the result of cluster in the dendrogram,in which the relative positions among all of the genotypes tested collected from different geographical distribution and climate factor were exhibited,there existed great relative correlations between activity of retrotransposons and ploidy as well as adaptive evolution in the genus of Diospyros.【Conclusion】The genotypes collected form south subtropical regions of China might be more ancient species.Retrotransposon might play an important role in ploidy evolution of this genus.
出处
《果树学报》
CAS
CSCD
北大核心
2012年第6期1004-1009,共6页
Journal of Fruit Science
基金
高等学校博士学科点专项科研基金项目(20110146110018)
国家自然科学基金(31000896)
江西省自然科学基金(20114BAB214005)
关键词
柿属植物
遗传关系
IRAP
逆转座子
Diospyros spp.
Genetic relationship
IRAP
Retrotransposon
