摘要
目的在云南省应用H1基因型麻疹野病毒鉴定方法,鉴别H1基因型麻疹野病毒感染病例,应用中国麻疹疫苗株病毒鉴定方法,鉴别接种麻疹疫苗后出现的麻疹样病例是否为麻疹疫苗相关病例。方法采用逆转录-聚合酶链反应-限制性片段长度多态性分析(Reverse Transcription-Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,RT-PCR-RFLP)方法、序列分析方法鉴别8株麻疹病毒。结果应用H1基因型麻疹野病毒RT-PCR-RFLP方法对8份标本进行鉴定,其中5份标本RT-PCR产物经SalI酶切后,被切成两个片段,分别为408个碱基对(base pair,bp)和159bp,显示5个病例是由H1基因型麻疹野病毒引起的。应用中国麻疹疫苗株病毒RT-PCR-RFLP方法对3份标本进行鉴定,其中1份标本RT-PCR产物经Af1II酶切,被切成两个片段,分别为287bp和151bp,显示该病例是由中国麻疹疫苗株病毒引起的。2份标本为非H1a基因亚型的麻疹野病毒。结论应用RT-PCR-RFLP方法对H1基因型麻疹野病毒和麻疹疫苗株病毒的鉴定结果,与麻疹病毒核蛋白(Nucleoprotein,N)基因测序分析的结果一致,可以作为快速鉴别H1基因型麻疹野病毒和麻疹疫苗株病毒的方法。
Objective Application the method to rapid identify the wild measles strain and China measles vaccine strain in Yunnan province. Method To apply method of reverse transcription-polymerase chain reaction-restriction fragment length polymorphism（RT-PCR-RFLP）and sequence analysis method to detect 8 strains of measles virus. Results The RT-PCR-RFLP method for H1 genotype was used for identifying 8 strains of measles virus, the 5 specimens of wild-type, measles viruses RT-PCR products were cut into 2 fragments, 408 bp and 159 bp,the results showed that 5 specimens of wild-type measles viruses were caused by H1 genotype. The RT-PCR-RFLP method for China vaccine strain identification was used for identifying 3 specimen of measles. 1 specimen RT-PCR products were cut into 2 fragments, 287 bp and 151 bp,which showed that this case was caused by China vaccine strain. 2 specimens of measles were non-H1 wild-type measles viruses. Conclusion The RT-PCR-RFLP method can be used to identify H1 genotype of measles virus and measles vaccine strain. The results are same as the method of nucleoprotein gene sequencing.
出处
《中国疫苗和免疫》
CAS
2012年第5期429-431,472,共4页
Chinese Journal of Vaccines and Immunization
