摘要
目的通过观察疾病活动指数(DAI)、组织病理学变化,研究白头翁汤对小鼠实验性溃疡性结肠炎的治疗作用,并进一步研究miR-19a的表达,探讨其治疗作用的可能机制。方法选取40只c57小鼠,随机等分为4组(10只/组):正常对照组、模型组(DSS)、治疗组(DSS+白头翁汤)和阳性对照组(DSS+5-ASA)。给予各模型组小鼠自由饮用3.5%DSS溶液以及相应药物灌胃,正常对照组自由饮用同等量的饮用水。每天观察并记录DAI评分,7 d后处死小鼠,取肠道炎症组织观察病理变化。RT-qPCR方法检测miR-19a表达变化。结果模型组小鼠的DAI评分及病理学评分显著高于对照组。DSS+白头翁汤组的DAI评分及病理学评分显著低于DSS模型组,说明白头翁汤对于小鼠实验性溃疡性结肠炎有一定的疗效。模型组miR-19a的表达显著低于对照组,DSS+白头翁汤组miR-19a的表达显著高于DSS模型组,提示白头翁汤可以通过增加miR-19a的表达而起到减轻炎症的作用。结论白头翁汤对于小鼠实验性溃疡性结肠炎有一定的疗效,可能通过增加miR-19a的表达而发挥作用。
Objective To investigate the therapeutic effect of Baitouweng Decoction on dextran sodium sulphate （DSS）-induced ulcerative colitis in mice and explore its mechanism involving miR-19a. Methods Forty female c57 mice were randomly allocated into 4 equal groups, namely the normal control group, model group （treated with 3.5% DSS solution）, treatment group （treated with DSS＋Baitouweng Decoction）, and positive control group （treated with DSS＋5-ASA）. Ulcerative colitis was induced in the mice by feeding them with 3.5% DSS in drinking water, and the mice in the control group were given water only. The disease activity index （DAI） of the mice in each group was recorded daily. Seven days later, the mice were sacrificed for histological examination of the intestines using HE staining; the expression of miR-19a mRNA in the intestines was detected using RT-qPCR. Results Compared with the control group, the model group showed significantly increased DAI and histological scores, and administration of Baitouweng Decoction significantly lowered the DAI and histological scores of the DSS-treated mice. The expression of miR-19a was lowered following DSS treatment, and Baitouweng Decoction treatment caused an increased miR-19a expression in DSS-treated mice. Conclusion Baitouweng Decoction has therapeutic effects on DSS-induced ulcerative colitis in mice, and this effect is probably mediated by enhancement of miR-19a expression in the intestines.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第11期1597-1599,共3页
Journal of Southern Medical University
基金
国家自然科学基金青年科学基金(81001506)
广东省中医药局科研课题计划(20112157)
广州中医药大学校级创新基金(10CX002)~~
