摘要
AIMS: Sulfated polysaccharide extracted from the brown algae Sargassum swartzii was studied for antioxidant potential. METHODS: The extracted sulfated polysaccharide was analyzed for physico-chemical characteristics, TAC, reducing power, free radical scavenging potentials (DPPH, ABTS, H2O2 radical) and antibacterial properties. RESULTS: The extract showed a high percentage of carbohydrate (7.40 ± 0.63) %, followed by sulfate (5.3 ± 1.54) %. The highest antioxidant activity was observed in ABTS (55 ± 3.61) %, followed by H2O2 (47.23 ± 2.81) % and DPPH (25.33 ± 2.52) %; significant differences were observed at (P ≥ 0.05). Among the ten human pathogenic strains tested, E. coli was the more sensitive. The characterization and mobility of the sulfated polysaccharide was examined by the FT-IR spectrum and assayed by agarose gel electrophoresis which showed highest mobility at higher pH buffer in carbonate-bicarbonate (pH 10) buffer. The molecular weight of the sulfated polysaccharide was determined by gradient PAGE and was found to be 50 KDa. Finally, GC-MS analysis revealed the presence of peaks corresponding to dimethyl-4-nitroaniline (26.34%). CONCLUSIONS: It is suggested that the sulfated polysaccharide from Sargassum swartzii could be a better source of natural antioxidant, as well as an antibacterial agent.
AIMS： Sulfated polysaccharide extracted from the brown algae Sargassum swartzii was studied for antioxidant poten- tial. METHODS： The extracted sulfated polysaccharide was analyzed for physico-chemical characteristics, TAC, reducing power, free radical scavenging potentials （DPPH, ABTS, H202 radical） and antibacterial properties. RESULTS： The extract showed a high percentage of carbohydrate （7.40 ± 0.63） %, followed by sulfate （5.3 ± 1.54） %. The highest antioxidant activity was observed in ABTS （55 ± 3.61） %, followed by H202 （47.23 ± 2.81） % and DPPH （25.33 ± 2.52） %; significant differences were observed at （P≥ 0.05）. Among the ten human pathogenic strains tested, E. coli was the more sensitive. The characterization and mobility of the sulfated polysaccharide was examined by the FT-IR spectrum and assayed by agarose gel electrophoresis which showed highest mobility at higher pH buffer in carbonate-bicarbonate （pH 10） buffer. The molecular weight of the sulfated polysaccharide was determined by gradient PAGE and was found to be 50 KDa. Finally, GC-MS analysis revealed the presence of peaks corresponding to dimethyl-4-nitroaniline （26.34%）. CONCLUSIONS： It is suggested that the sulfated polysaccharide from Sargassum swartzii could be a better source of natural antioxidant, as well as an antibacterial agent.
出处
《中国天然药物》
SCIE
CAS
CSCD
2012年第6期421-428,共8页
