摘要
目的测定大鼠单侧输尿管部分梗阻(PUUO)后输尿管平滑肌细胞中M3受体mRNA的转录水平和蛋白表达水平改变的规律,以探讨PUUO后输尿管平滑肌细胞中M3受体的作用。方法 Wistar雄性大鼠80只,随机分为4组:8周PUUO组、16周PUUO组、8周对照组及16周对照组,每组各20只。两PUUO组(n=40)采用把大鼠输尿管左侧上1/2段埋入腰大肌做成PUUO的大鼠模型,两对照组(n=40)只游离左侧输尿管。模型制作成功后于8周和16周时取出输尿管,分别用RT-PCR检测输尿管平滑肌M3受体mRNA的含量,Western-blotting检测输尿管平滑肌M3受体蛋白的含量。结果 RT-PCR结果示:M3受体mRNA的表达8周PUUO组高于8周对照组(55.16±3.94)vs(36.48±3.87),16周PUUO组低于16周对照组(19.37±3.70)vs(37.39±3.65),8周PUUO组高于16周PUUO组,差异均有统计学意义(均P<0.05)。Western-blotting结果示:M3受体蛋白的表达8周PUUO组高于8周对照组(27.13±1.77)vs(21.36±2.69),16周PUUO组低于16周对照组(19.41±1.37)vs(23.43±1.58),差异均有统计学意义(均P<0.05),8周对照组与16周对照组比较差异无统计学意义(P>0.05)。结论梗阻8周时大鼠输尿管平滑肌中M3受体的生物合成上调,梗阻至16周时输尿管平滑肌中M3受体生物合成下调,为后续与临床相结合的研究做了更进一步的铺垫。
Objective To investigate the changing regulation of M 3 receptor in mRNA and protein in smooth muscle cells in rats after partial unilateral ureteral obstruction（PUUO）.Methods 80 male Wistar rats were divided into 4 groups randomly：the 8-week PUUO group（n=20）,8-week control group（n=20）,16-week PUUO group（n=20） and 16-week control group（n=20）.The upper half section of left ureters（n=40） in the two PUUO groups were embedded into the psoas muscles,while the rats in the two control groups（n=40） were only received laparotomy.The ureters were cut at different experimental time（the 8th week and the 16th week separately） after the models were established.Then,the mRNA and the protein expression of M 3 receptor were detected by the RT-PCR and Western-blotting.Results According to the results of RTPCR,the mRNA expression level of M 3 receptor in 8-week PUUO group was higher than that of 8-week control group（55.16±3.94） vs（36.48±3.87）,the 16-week PUUO group was lower than 16-week control group（19.37 ±3.70） vs（37.39± 3.65）,8-week PUUO group was higher than that of the 16-week PUUO group,the differences were all statistically significant（all P0.05）.According to the results of Western blotting experiments,the M 3 receptor protein expression level in the 8-week PUUO group was higher than that of 8-week control group（27.13±1.77） vs（21.36±2.69）,the 16-week PUUO group was lower than 16-week control group（19.41±1.37） vs（23.43±1.58）,the differences were all statistically significant（all P0.05）,the difference between the 8-week control group and 16-week control group was no statistical significance（P0.05）.Conclusion The series of experiments demonstrate an increase of M 3 receptor in smooth muscle cells in PUUO rats at 8 weeks while a decrease of that at 16 weeks,which provides a foreshadowing of the clinical researchfor the next step.
出处
《中国医药导报》
CAS
2012年第29期34-36,共3页
China Medical Herald
基金
山西省国际科技合作项目(项目名称:输尿管梗阻的上尿路动力学改变的研究
项目编号:2007081036)
关键词
单侧输尿管部分梗阻
输尿管平滑肌
M3受体
Partial unilateral ureteral obstruction
Ureteral smooth muscle
M3 receptor
