摘要
目的研究骨水泥(聚甲基丙烯酸甲酯,PMMA)栓子致兔急性肺栓塞后细胞因子(TNF-α、IL-6)水平变化及其意义。方法取成年健康日本大耳白兔24只,随机分为骨水泥栓塞组、自体血凝块栓塞组、对照组,每组8只,栓塞组经兔股静脉分别注入骨水泥栓子、自体血凝块栓子,制备肺血栓栓塞症(PTE)模型,并动态观察TNF-α、IL-6的变化。结果骨水泥栓子注入后TNF-α在0.5～6.0h呈逐渐升高趋势,与同期对照组、血凝块栓塞组比较差异均有统计学意义(P<0.05)。IL-6在0.5h与对照组、血凝块栓塞组比较差异无统计学意义(P>0.05),1～6h与同期对照组、血凝块栓塞组比较差异均有统计学意义(P<0.05)。血凝块栓塞组与同期对照组在各时点的TNF-α、IL-6比较,差异均无统计学意义(P>0.05)。结论单枚血凝块栓子致兔PTE后,在早期(6h内)未见TNF-α、IL-6的高表达,而骨水泥栓塞组引起兔血清TNF-α、IL-6升高。
Objective To investigate the change and significance of cytokine levels in acute pulmonary thromboembolism induced by bone cement in rabbits.Methods Twenty-four rabbits were randomly devided into 3 groups,8 rabbits were established pulmonary embolism model by injection of bone cement embolus into femoral vein,8 rabbits were injected autol ogous blood clot into femoral vein,another 8 rabbits as control group.Then the dynamical changes of tumor necrosis fac tor-α（TNF-α） and interleukin-6（IL-6） in blood were observed.Results TNF-α in blood were increased significantly after injection of bone cement embolus 0.5 to 6 hours,there was statistically significant difference between bone cement em bolus group and the both blood clot group and control group（P 0.05）.There was no statistically significant difference about IL-6 in 0.5 h with the control group,blood clot embolization group（P 0.05）;for 1-6 h with the same period in the control group,the blood clot embolization group,difference was statistically significant（P 0.05）.There was no statistically significant difference about TNF-α,IL-6 in blood clot embolization group with the same period in the control group at each time point（P 0.05）.Conclusion TNF-α,IL-6 in blood circulation is not significantly elevated in 6 h after PTE induced by one blood clot embplus,so it is bone cement to induce the changes of TNF-α,IL-6 in blood circulation.
出处
《中国当代医药》
2012年第29期10-11,14,共3页
China Modern Medicine
