摘要
目的:建立复方刺五加颗粒的质量标准。方法:采用薄层色谱法对方中的西洋参、麦冬、黄芪进行定性鉴别;采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定方中黄芪甲苷的含量。结果:薄层色谱斑点清晰,分离效果较好,且阴性无干扰;黄芪甲苷在0.56～5.60μg范围内呈良好的线性关系(r=0.9997),平均回收率为99.13%,RSD为0.56%(n=5)。结论:该方法准确可靠、专属性强、重现性好,可用于复方刺五加颗粒的质量控制。
Objective：To establish the quality standard of Fufang Ciwujia Granules. Method：TLC method was used to identify American Cinseng, Dwarf Lilyturf Root Tuber and Mongolian Milkvetch Root ; HPLC methed was used to determine the Astragaloside IV in Mongolian Milkvetch Root. Result：The TLC spots were clear without interference of the negative control ;Astragaloside IV was linear in the range of 0.56 -5.60μg（ r = 0. 9997 ）, and the average recovery was 99.13 % with RSD 0.56% （ n = 5 ）. Conclusion ： This method is accurate, reliable , specific and reproducible, and can be used for quality control of Fufang Ciwujia Granules.
出处
《中华中医药学刊》
CAS
2012年第11期2571-2573,I0007,共4页
Chinese Archives of Traditional Chinese Medicine
