摘要
锌指蛋白基因是植物基因组中最大最复杂的基因家族之一.大部分的锌指模体存在于转录因子中,它们在转录水平上参与植物生长发育及植物对生物和非生物胁迫的反应.为了解锌指蛋白基因在水稻种子发育中的作用,本研究通过多种数据库搜索获得了878个水稻锌指蛋白基因.从中选取311个利用RT-PCR技术分析它们在水稻成熟期根、茎、叶、花及不同发育阶段种子中的表达特征.结果发现,共有196个基因能在至少1个水稻器官中表达,其中10个为种子特异性表达基因.进一步分析发现,10个特异表达基因在水稻种子不同发育阶段中的表达具有种子阶段表达特异性.同时分析它们的基因及蛋白结构特点,结果显示它们的结构较简单,其中3个蛋白含有线粒体靶肽,5个蛋白含有CCCH锌指结构域.另外,分析种子特异性表达基因上游调控区的顺式作用元件,结果表明它们都含有TATA-box、CAAT-box和种子特异调控元件,除此之外还发现了光、激素和胁迫反应相关调控元件.这些结果为进一步研究它们在种子发育过程中的生物学功能提供了有用的线索.
Zinc finger protein genes constitute one of the largest and most complex gene families in the plant genomes.Most classes of zinc finger motifs are present in transcription factors,which have been described to respond to plant growth development and biotic and abiotic stresses at the transcriptional level.To elucidate the functions of the zinc finger protein genes on seed development processing in rice,we collected 878 rice zinc finger protein genes and their sequences from some related databases.By RT-PCR technique,311 genes of them were selected to analyze expression profiles during rice ripening phase,including mature root,stem,leaf,flower and seeds at different developmental stages.The results were found that 196 zinc finger genes were detected products of gene expression at least in one tested organs in rice and 10 of them were specifically or preferentially transcribed in seeds.Further analyzing the expression of ten specific genes at different stages of rice seed development indicated that they had seed-stage specific expression.Meanwhile,analysis on the structure of the genes and proteins showed that their structures were simple,three of which had mitochondrial targeting peptide and five proteins had CCCH zinc finger domains.In addition,analysis of the cis-elements showed that promoter regions of the seed-specific genes not only contained TATA-box,CAAT-box and seed-specific elements,but also contained other elements that were mainly associated with light,hormone and stress responses.Taken together,the results provided informative clues for further studies about their functions in the complex developmental processes of rice seeds.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2012年第11期1033-1039,共7页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金项目(No.30370769)
福建省自然科学基金项目(No.2009J01091)
福建省财政专项-福建省农业科学院科技创新团队项目(No.CXTD2011-09)~~
关键词
水稻
锌指蛋白基因
种子特异表达
RT-PCR
rice（Oryza sativa）
zinc finger protein genes
seed-specific expression
RT-PCR
