摘要
目的 探讨神经元型一氧化氮合酶 (neuronalnitricoxidesynthase,nNOS)及一氧化氮 (nitricoxide ,NO)在学习记忆机制中的相关作用。方法 采用免疫组化方法观察Y迷宫空间辨别学习训练后大鼠不同脑区nNOS表达变化 ,并探讨特异性nNOS抑制剂 7 nitroindozal(7 NI)、钙拮抗剂尼莫通 (nimotop)腹腔注射对大鼠学习获得和记忆再现能力的影响。结果 学习训练后海马各亚区nNOS样神经元数量及染色强度明显增加 ,而皮层和纹状体区则无显著变化 ;7 NI以剂量依赖方式损伤大鼠的学习获得能力 ,但不影响记忆再现 ,尼莫通则对这两种能力均有破坏。结论 提示学习记忆过程可能伴有nNOS合成及活性增加 ,nNOS/NO在学习获得阶段具有重要作用。
Objective The aim of the present study was to elucidate the possible role of neuronal nitric oxide synthase (nNOS) and nitric oxide (NO) in learning and memory mechanism. Methods nNOS was assayed immunohistochemically in different brain regions of rats in normal control,sham training and Y maze spatial learning groups,also the acquisition and retrieval of rats in Y maze tasks were examined following treatment with 7 NI,a selective nNOS inhibitor,or Ca 2+ antagonist nimotop. Results The number of nNOS containing neurons and its immunoreactivity in CA 1,CA 2 3 and dentate gyrus,but not in striatum and cortex,increased after Y maze training.7 NI produced significant and dose dependent acquisition deficits,which could be retrieved by L Arg,but failed to affect the retrieval in maze tasks.In contrast to 7 NI,nimotop could damage both acquisition and retrieval significantly. Conclusion The results indicate that the synthesis and activity of nNOS are increased during the processing of learning and memory.nNOS/NO may play an important role in acquisition phase ,but not retrieval of learning and memory.
出处
《中国神经免疫学和神经病学杂志》
CAS
2000年第2期116-122,共7页
Chinese Journal of Neuroimmunology and Neurology
关键词
一氧化氮
一氧化氮合酶
学习记忆
nitric oxide
nitric oxide synthase
learning and memory
