摘要
目的探讨双歧杆菌三联活菌对大鼠急性肝损伤肠源性内毒素血症是否具有保护作用。方法 24只SD雄性大鼠随机分为正常对照组、肝损伤组及双歧杆菌三联活菌组3组,每组各8只。肝损伤组及双歧杆菌三联活菌组大鼠采用皮下注射硫代乙酰胺(TAA)诱导急性肝损伤,双歧杆菌三联活菌组大鼠以双歧杆菌三联活菌灌胃进行干预,正常对照组不予任何处理。采用动态浊度法检测各组大鼠血浆内毒素水平;采用赖氏法检测血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)水平,采用标准法检测血清乳酸脱氢酶(LDH)水平,采用TBA法检测血清丙二醛(MDA)水平。HE染色观察各组大鼠肝组织病理学改变,测定肝组织损伤面积百分比;TUNEL法检测各组大鼠肝组织肝细胞凋亡,计算凋亡率。结果 (1)肝损伤组大鼠血浆内毒素、血清ALT、AST、LDH、MDA水平较正常对照组大鼠明显增高,且差异有统计学意义(t值分别为10.100、8.891、3.516、8.812、4.309,P均<0.01)。双歧杆菌三联活菌组大鼠血浆内毒素水平较肝损伤组大鼠明显降低,且差异也有统计学意义(t=9.292,P<0.01);而双歧杆菌三联活菌组大鼠血清ALT、AST、LDH、MDA水平与肝损伤组大鼠比较差异无统计学意义。(2)肝损伤组大鼠肝组织损伤面积百分比大于正常对照组大鼠,且差异有统计学意义(t=13.569,P<0.01);双歧杆菌三联活菌组大鼠肝组织损伤面积百分比小于正常对照组大鼠,且差异也有统计学意义(t=24.876,P<0.01)。(3)普通光镜和荧光显微镜下,肝损伤组大鼠肝细胞凋亡率均高于正常对照组大鼠,且差异均有统计学意义(t值分别为6.506、6.249,P均<0.01);而双歧杆菌三联活菌组大鼠肝细胞凋亡率均低于肝损伤组大鼠,且差异也均有统计学意义(t值分别为5.006、4.771,P均<0.01)。结论双歧杆菌三联活菌可改善急性肝损伤大鼠肠道菌群失调,降低血浆内毒素水平,对急性肝损伤�
Objective To identify the protective effects of Bifid triple viable on intestinal endotoxemia in rats with acute liver injury. Methods Twenty-four SD rats were randomly divided into three groups： normal control group （N）, model group （T） and Bifid triple viable group （P）. The model of acute liver injury was induced by subcutaneous injections of thioacetamide （TAA）. The levels of endotoxin, serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, lactate dehydrogenase （LDH）, malondialdehyde（MDA） were measured by biochemical method. Histopathology of liver were observed by HE staining. And hepatocyte apoptosis was detected by TdT-mediated dVTP nick end labeling （TUNEL）. Results （ 1 ） Compared with group N, the content of endotoxin, ALT, AST, LDH, MDA significantly increased in group T（t = 10. 100,8. 891,3.516,8. 812,4. 309, P 〈0. 01 ）. Compared with group T, the levels of plasma endotoxin decreased significantly in group P（t = 9. 292,P 〈 0. 01 ）. But compared with group T, the content of ALT, AST, LDH, MDA was not significantly increased in group Bifid triple viable. （2） Compared with group N, the percentage of the liver damage significantly increased in group T（ t = 24. 876 ,P 〈 0. O1 ）.The percentage of the liver damage were lesser in group P than that in group T（ t = 13. 569, P 〈 0.01 ）. （3） Under ordinary light microscope and fluorescence microscope, compared with group N, the hepatic apoptosis index significantly increased in group T（ t = 6. 506,6. 249, P 〈 0.01 ）. The hepatic apoptosis index were lesser in group P than those in group T （ t = 5. 006,4. 771, P 〈 0. 01 ）. Conclusion Bifid triple viable protects rats from acute liver injury by improving intestinal dysbacteriosis and reducing the level of plasma endotoxin.
出处
《中华消化病与影像杂志（电子版）》
2012年第2期5-8,共4页
Chinese Journal of Digestion and Medical Imageology(Electronic Edition)
