摘要
目的:利用刀豆蛋白A(ConA)诱导建立小鼠肝炎模型,观察淫羊藿苷对该损伤模型保护作用的细胞免疫学和分子免疫学机制。方法:采用C57BL/6雄性小鼠,随机分为淫羊藿苷+ConA组、生理盐水+ConA组、淫羊藿苷+生理盐水组。小鼠尾静脉注射ConA,建立T细胞介导的免疫性肝脏损伤模型;采用转氨酶试剂盒测定各组小鼠血液中转氨酶含量;组织病理学观察实验小鼠肝脏组织和肝细胞坏死变化;采用ELISA试剂盒测定血清中炎性细胞因子IFN-γ、TNF-α含量;流式细胞术观察注射ConA后肝脏淋巴细胞的活化变化。结果:与ConA对照组相比,淫羊藿苷干预组小鼠血液中ALT和AST水平明显降低,P<0.01;IFN-γ、TNF-α含量明显降低,P<0.05;H&E染色可见淫羊藿苷干预组小鼠肝细胞核完整,未见炎性细胞的浸润,而ConA对照组小鼠肝细胞核固缩,部分核膜破裂,肝组织内有炎症细胞和红细胞浸润;流式细胞技术发现淫羊藿苷可明显延缓由ConA引起的肝脏NKT细胞的活化,减弱T细胞的浸润。结论:淫羊藿苷对ConA诱导的小鼠肝脏损伤有明显的保护作用,其机理可能与淫羊藿苷降低血液中IFN-γ、TNF-α表达及影响肝脏NKT细胞的活化有关。
Objective：To explore the protection effects and mechanisms of Icariin in mice liver injury model induced by concanavalin A（ConA）.Methods：Mice liver injury models were established with ConA by tail vein injection;ALT and AST levels in serum were detected by a transaminase kit;necrotic areas and mononuclear cells of the mice＇s liver were observed by hematoxylin/eosin staining;IFN-γ and TNF-α levels in serum were measured with ELISA kits.Activity of hepatic lymphocytes was tested with FACS.Results：Compared with mice treated with ConA alone,serum ALT and AST levels of C57BL/6 mice pretreated with Icariin were reduced significantly,P〈0.01;IFN-γ and TNF-α levels were also decreased manifestly,P〈0.05;The apoptosis and necrosis areas in liver of Icariin-pretreated mice were obviously reduced,only few infiltrating lymphocytes were found;the activity of liver NKT cells was postponed by Icariin.Conclusion：Icariin can protect mice liver from injury induced by ConA,and the protection mechanisms may relate to reduce the secretion levels of IFN-γ and TNF-α and to affect liver NKT cells activity.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第10期900-903,共4页
Chinese Journal of Immunology
基金
山东省人力资源与社会保障厅留学归国人员基金项目(No.201178006)
