摘要
目的研究小鼠成骨前体细胞MC3T3-E1在自组装多肽水凝胶材料上黏附、伸展、增殖和分化的能力。方法人工合成短肽精-丙-天-丙16(RADA16),制备RADA16水凝胶并用扫描电镜观察其微观结构。同时在RADA16多肽水凝胶支架材料上接种MC3T3-E1细胞,观察细胞黏附、伸展、增殖和分化的情况。结果 MC3T3-E1细胞能够在多肽水凝胶上黏附、伸展和增殖。成骨诱导培养后的细胞有较高水平的ALP、OC表达及矿化基质沉积,成骨相关基因Runx2,OSX,AKP-2和OC也有高表达,且表达量随着时间的延长而不断升高。结论自组装多肽水凝胶能够支持MC3T3-E1细胞的黏附、伸展、增殖和分化,显示了良好的生物相容性,可能在牙周炎所致的骨质缺损修复中有广泛应用。
Objective To investigate the ability of attachment, spreading, proliferation and differentiation of mouse pre - osteo- blasts, MC3T3 - E1, on RADA16 self - assembling peptide hydrogel. Methods RADA16 peptide was custom synthesized. RADA16 peptide hydrogel was prepared and SEM was used to explore its ultra -structure. Then, MC3T3 -E1 cells were seeded on RADA16 pep- tide hydrogel. Cell attachment, cell spreading, cell proliferation and cell differentiation were evaluated. Results MC3T3 - E1 cells ad- hered and spread well on RADA16 peptide hydrogel. High ALP activity, OC production, calcium depositon and expressions of bone - re- lated genes （Runx2, OSX, AKP -2, OC） were detected, and their expressions were increased with the culture time. Conclusion Self - assembling peptide scaffold can support pre - osteoblast attachment, spreading, proliferation and differentiation and show good compati- bility. This kind of peptide scaffold might have a wide application in dentistry, especially the treatment of bone defects caused by periodon- tal disease.
出处
《医学研究杂志》
2012年第10期72-75,共4页
Journal of Medical Research
基金
2007年浙江省科技厅基金资助项目(2007C 23016)
