摘要
较小鼠等啮齿类动物而言,猴和小型猪等大型实验动物在亲缘关系上与人类更为接近,在解剖、生理生化代谢及疾病发病机制等多方面与人类更接近,使它们在复制人类疾病模型,研究疾病发病机制和新药研发等中有无可替代的应用。而制备遗传工程大动物可以更深入地解析人类疾病,并可为器官移植和新药研发提供更充分的实验材料。基于慢病毒介导的转基因方法近几年已越来越多地被用来制备遗传工程猴和小型猪。与传统的原核显微注射方法和体细胞核移植法相比,慢病毒介导的转基因方法转基因效率高,操作更简单。因此,构筑基于慢病毒介导的转基因方法制备遗传工程猴和小型猪的技术平台将对生物医学研究产生巨大推动作用。
Compared with the rodents such as mice,large laboratory animals（i.e.,minipigs and monkeys） have more similarities in size,anatomy,physiology,biochemistry,metabolism and disease pathogenesis with humans.Therefore,they may be widely employed and play important roles in replicating human disease models,studying disease pathogenesis and drug discovery,etc.In recent years,lentivirus-mediated gene delivery approach is increasingly used to produce genetically engineered monkeys and minipigs.Compared with pronuclear microinjection and somatic cell nuclear transfer,lentivirus-mediated gene delivery used to generate transgenic animals enjoys the high transgenic efficacy and simple operation.Therefore,it is quite necessary to set up a technology platform for efficiently producing genetically engineered monkeys and minipigs by lentivirus-mediated gene delivery.
出处
《中国实验动物学报》
CAS
CSCD
2012年第5期84-89,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家973项目(No.2011CBA01006)
广州市科技计划项目(No.2009A1-E051)
国家自然科学基金项目(No.81172587)
广东省科技计划项目(No.9151063101000015)
关键词
小型猪
猴
遗传工程动物
慢病毒载体法
原核显微注射法
体细胞核移植
Minipig
Monkey
Genetically engineered animals
Lentivirus-mediated gene delivery
Pronuclear microinjection
Somatic cell nuclear transfer
