摘要
低分子肝素是由商品肝素分得的低分子组分,或利用化学解聚法等将其裂解制得。本实验以肝素生产的副产品——低抗凝肝素为原料,经亚硝酸控制解聚和凝胶层析制备低分子肝素。
<Absrtact>Low molecular weight heparin (LMWH) can ba prepared by either fractionation or chemical depoly-merization of commercial heparin. We prepared LMWH from low anticoagulant activity heparin (LAAH), a side product accompanying commercialhe-parin production, by depolymerization with nitrous acidcombined with gel chromatography·; Its anticoag-ulant、 activity,physical and chemical parameters were determined. The ntithrombotic activity was eva-luated by the formation of thrombus in common ca-rotid artery in experimental rat thrombus model. The results showed that the anticoagulant ac-tivity of LMWH obtained was 30.33 a/mg, the eve-rage molecular weight was 5200d. The content ofglucosamine, hexuronic acid and sulfate was lowerthan that of LAAH. This implied that certain struc-ture changes had occurred, however, the UY and IRspectra of LMWH suggested that its main structure remained intact.The antithrombotic action of LMWH was stron-ger than LAAH and commercial heparin and theaction was dose dependent.LMWH obtained from LAAH not only costs less. but also might have more favorable clinical potential.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1990年第4期178-178,共1页
Chinese Journal of Pharmaceuticals
