摘要
目的探索枸杞多糖(Lycium barbarum polysaccharides,LBP)对小鼠移植性肝癌端粒酶活性及其限速酶hTERT表达的影响。方法采用皮下接种法建立小鼠移植性肝癌模型,将96只小鼠随机分成正常对照组,模型组,LBP高、中、低(20,10,5 mg/kg·d)剂量组和环磷酰胺(20 mg/kg·d)组。观察LBP对小鼠肿瘤体积、抑瘤率的影响。PCR-TRAP-ELISA法检测端粒酶的活性,Western blot测定hTERT蛋白表达。结果 20,10,5 mg/kg·d的LBP能明显减小肿瘤细胞的体积和质量(P<0.05),抑瘤率分别为42.23%、25.10%和9.16%;同时,LBP(20,10mg/kg·d)能明显抑制肝癌组织hTERT蛋白表达(P<0.05),降低端粒酶的活性(P<0.05)。结论 LBP可通过抑制小鼠肝癌组织hTERT蛋白表达、降低端粒酶的活性,从而对小鼠移植性肝癌的生长起到抑制作用。
Objective To explore the effect of Lycium barbarum polysaccharides on the telomerase activation and hTERT expres- sion on the portability liver cancer in mice. Methods The portability liver cancer models were established with hypodermic inocu- lation. Ninety - six mice were randomly divided into 6 groups：normal control group, model group, LBP high, middle,low - dose group, positive control group. To observe the effect of LBP on the gross tumor volume, inhibition rates of tumor growth. Telomerase activation were determined by PCR -TRAP- ELISA, hTERT expression were determined by Western blot. Results LBP（20mg/ kg · d, 10mg/kg · d, 5mg/kg · d） could obviously depress the gross tumor volume and weight（P 〈 0.05）. The difference of in- hibition rates of tumor growth were 42.23% ,25.10% and 9.16qv. LBP（20mg/kg · d、10mg/kg · d） could obviously inhibit the hTERT expression and depress the telomerase activation in liver cancer （ P 〈 0.05 ）. Conclusion LBP can produce depressant effects on the portability liver cancer in mice. The effects concerned with the LBP can inhibit the hTERT expression and depress the telomerase activation.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第10期2438-2440,共3页
Lishizhen Medicine and Materia Medica Research
基金
宁夏回族自治区自然科学基金(No.NZ1092)
宁夏医科大学科研项目(No.XM2011002)
关键词
枸杞多糖
H22肝癌细胞
端粒酶
HTERT
Lycium barbarum polysaccharide, H22 hepatoma carcinoma cell
Telomerase, hTERT
