摘要
为了探究枣黄化根蘖苗的成因,对其黄化根蘖苗、正常根蘖苗和母株之间的形态学指标、叶绿素及矿质元素含量和相关基因表达水平进行了分析。结果表明,与正常植株比,黄化根蘖苗形态学指标没有明显变化,但叶绿素含量及叶绿素a/b比值显著降低,矿质元素N、P、K、Mn、Cu、Zn、Fe含量显著增高,但Ca和Mg差异不显著。同时,AFLP分析表明,黄化根蘖苗与正常植株在基因组水平没出现显著差异;但cDNA-AFLP和RT-PCR分析表明在转录水平上,黄化根蘖苗中酰基转移酶基因表达增加,Ty3-gypsy反转录转座子基因和GAGA结合转录激活因子基因表达降低。综合分析认为,枣根蘖苗的黄化可能是与叶绿素合成转录相关的反转录转座子和GAGA结合转录激活因子基因表达降低,影响了叶绿素合成,使植株最终表现黄化;同时黄化植株中与光呼吸相关的一些基因,如酰基转移酶基因表达相应增加以适应这种逆境胁迫,说明这些基因间存在着相互适应的调控网络。
In order to explore the reason of etiolated seedling from root suckers of Chinese jujube, morphological index,chlorophyll content,mineral content and molecular variation of etiolated and normal seedling from root suckers of jujube trees were studied in this paper. The results showed that there was no significant change in morphological index between etiolated seedling and healthy seedling from root suckers,but the chlorophyll content of etiolated seedling was significantly lower than that of healthy ones, the mineral content of N,P,K,Mn,Cu,Zn and Fe were significantly increased,Ca and Mg were no significant change. The AFLP analysis showed that there were no obvious differences on the level of genome between etiolated seedling from root suckers and healthy plants. The results of cDNA-AFLP and RT-PCR showed that the Acyltransfer gene expression was higher in etiolated seedlings compared to healthy ones,expression of Ty3-gypsy was decreased dramatically and the expression of GAGA-binding transcriptional activator was reduced slightly in etiolated ones. So the change of expression of GAGA- binding transcriptional activator and Ty3-gypsy may influence the chlorophyll synthesis,and then resulted in the etiolation. But in that stress case the expression of genes related photorespiration,such as Acyltransfer,were increased. Those results showed that there were regulatory networks among those genes.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第10期1919-1927,共9页
Acta Horticulturae Sinica
基金
河北省自然科学基金项目(C2009000534
08D020)
河北省杰出青年基金项目(C2010000679)
国家科技支撑计划项目(2008BAD92B03
2007BAD36B07
2006BAD18B02)
