摘要
从盐碱地菊芋根际土壤中筛选出112株产菊粉酶菌株,并从中得到一株酶活较高的真菌A-6,经菌落观察以及采用真菌通用引物ITS1和ITS4 PCR扩增RNA基因序列,测序鉴定结果为烟曲霉Aspergillus fumigatus。对其产酶条件进行单因素分析结果显示,真菌A-6的pH耐受范围较广,且具有一定的耐盐性。经正交实验进行产酶条件优化,确定了A-6的最佳产酶条件为菊芋提取液(EJA)100g/L,酵母膏(YE)25g/L,NaCl为5g/L,NH4H2PO45g/L,pH为5,30℃,170r/min下振荡培养3d,酶活最高为13.29U/mL。
112 strains producing inulinase were isolated from rhizosphere soil of Jerusalem artichoke in the salt- affected land.Strain A-6 was obtained and identified as Aspergillus fumigatus through observing its shape of individual bacterial colony and amplified the rRNA gene sequence using fungal universal primers ITS1 and ITS4. The effect of different factors on its enzyme production shown A-6 had a wide pH range and it could tolerate the high concentration of NaCI. After analysis of orthogonal design, the optimum condition for higher inulinase production of A-6 was determined. EJA 100g/L, YE 25g/L, NaCl 5g/L, NH4H2PO45g/L, pH5,3d of growth under 30℃ and 170r/min in shake cultures.Maximal inulinase activity of A-6 was 13.29U/mL.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第21期160-163,共4页
Science and Technology of Food Industry
基金
国家科技支撑项目(2011BAD13B09)
江苏省科技支撑项目(BE2010305和BE2011368)
公益性行业(农业)科研专项经费项目(200903001-05)
中央高校基本业务费项目(Y0201100249)
教育部博士点基金(20100097120016)
关键词
筛选
菊粉酶
烟曲霉
产酶条件
优化
screening
inulinase
Aspergillus fumigatus
fermentation condition
optimizing
