摘要
狂犬病病毒为不分节段单链负股的RNA病毒,属弹状病毒科狂犬病病毒属。世界上几乎所有国家都有狂犬病发生,狂犬病病毒能够使所有温血动物发病致死,死亡率高达100%。本研究根据GenBank中的狂犬病病毒M基因序列,选择保守区域设计引物,通过对SYBR-GreenⅠ实时荧光PCR反应条件进行优化,建立了用于检测狂犬病病毒的SYBR-GreenⅠ实时荧光PCR方法。结果显示,建立的狂犬病病毒实时荧光定量PCR方法,具有特异性强、灵敏度高、重复性好的优点,是开展狂犬病的临床检测的有力工具。
Rabies virus is a non-segmented, negative-strand RNA virus, belonging to the genus of lyssavirus of the rhabdoviridae family. Rabies distributes all over the world, all warm-blood animal are susceptible to rabies virus with 100% mortality rate. In this study, the conserved regions of rabies virus M genes chosen from GenBank were used to design primers for fluorescent quantitative PCR, after optimized the reaction condition, the SYBR-GreenⅠfluorescent quantitative PCR method for detecting rabies virus was established and used to detect rabies virus. The results showed SYBR-GreenⅠfluorescent quantitative PCR was high specific, high sensitivity, good reproducible, which might be a useful tool for detecting rabies virus.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2012年第5期441-446,共6页
Genomics and Applied Biology
基金
国家重点基础研究发展计划(973计划)
狂犬病毒分子流行病学及种间进化特点课题(2005CB523000)资助
